| Mechanical loading affects the energy metabolism of intervertebral disc cells. | |
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MedLine Citation:
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PMID: 21484859 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Research has shown that mechanical loading affects matrix biosynthesis of intervertebral disc (IVD) cells; however, the pathway(s) to this effect is currently unknown. Cellular matrix biosynthesis is an energy demanding process. The objective of this study was to investigate the effects of static and dynamic compressive loading on energy metabolism of IVD cells. Porcine annulus fibrosus (AF) and nucleus pulposus (NP) cells seeded in 2% agarose were used in this experiment. Experimental groups included 15% static compression and 0.1 and 1 Hz dynamic compression at 15% strain magnitude for 4 h. ATP, lactate, glucose, and nitric oxide (NO) contents in culture media, and ATP content in cell-agarose construct were measured using biochemical assays. While the total ATP content of AF cells was promoted by static and dynamic loading, only 1 Hz dynamic loading increased total ATP content of NP cells. Increases in lactate production and glucose consumption of AF cells suggest that ATP production via glycolysis is promoted by dynamic compression. ATP release and NO production of AF and NP cells were significantly increased by dynamic loading. Thus, this study clearly illustrates that static and dynamic compressive loading affect IVD cell energy production while cellular responses to mechanical loading were both cell type and compression type dependent. |
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Authors:
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Hanan N Fernando; Jessica Czamanski; Tai-Yi Yuan; Weiyong Gu; Abdi Salahadin; Chun-Yuh Charles Huang |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-04-11 |
Journal Detail:
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Title: Journal of orthopaedic research : official publication of the Orthopaedic Research Society Volume: 29 ISSN: 1554-527X ISO Abbreviation: J. Orthop. Res. Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-09-28 Completed Date: 2011-11-18 Revised Date: 2013-02-19 |
Medline Journal Info:
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Nlm Unique ID: 8404726 Medline TA: J Orthop Res Country: United States |
Other Details:
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Languages: eng Pagination: 1634-41 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Orthopaedic Research Society. |
Affiliation:
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Stem Cell and Mechanobiology Laboratory, Department of Biomedical Engineering, College of Engineering, University of Miami, Coral Gables, Florida 33146-0621, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism Animals Bioreactors Cell Survival / physiology Compressive Strength / physiology Culture Media / pharmacology Energy Metabolism / physiology* Extracellular Matrix / metabolism, physiology Glucose / pharmacokinetics Intervertebral Disc* / cytology, metabolism, physiology Nitric Oxide / metabolism Sepharose / pharmacology Sus scrofa Weight-Bearing / physiology* |
| Grant Support | |
ID/Acronym/Agency:
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AR056101/AR/NIAMS NIH HHS; EB008653/EB/NIBIB NIH HHS; R01 EB008653-01A1/EB/NIBIB NIH HHS; R03 AR056101/AR/NIAMS NIH HHS; R03 AR056101-01A1/AR/NIAMS NIH HHS; R03 AR056101-02/AR/NIAMS NIH HHS; R03 AR056101-03/AR/NIAMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 10102-43-9/Nitric Oxide; 50-99-7/Glucose; 56-65-5/Adenosine Triphosphate; 9012-36-6/Sepharose |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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