| Mechanical load inhibits IL-1 induced matrix degradation in articular cartilage. | |
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MedLine Citation:
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PMID: 19747586 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Osteoarthritis is a disease process of cellular degradation of articular cartilage caused by mechanical loads and inflammatory cytokines. We studied the cellular response in native cartilage subjected to a mechanical load administered simultaneously with an inflammatory cytokine interleukin-1 (IL-1), hypothesizing that the combination of load and cytokine would result in accelerated extracellular matrix (ECM) degradation. METHODS: Mature bovine articular cartilage was loaded for 3 days (stimulation) with 0.2 and 0.5 MPa stresses, with and without IL-1 (IL-1alpha, 10 ng/ml), followed by 3 days of no stimulation (recovery). Aggrecan and collagen loss were measured as well as aggrecan cleavage using monoclonal antibodies AF-28 and BC-3 for cleavage by aggrecanases (ADAMTS) and matrix metalloproteinases (MMPs), respectively. RESULTS: Incubation with IL-1 caused aggrecan cleavage by aggrecanases and MMPs during the 3 days of stimulation. A load of 0.5 MPa inhibited the IL-1-induced aggrecan loss while no inhibition was found for the 0.2 MPa stress. There was no collagen loss during the treatments but upon load and IL-1 removal proteoglycan and collagen loss increased. Load itself under these conditions was found to have no effect when compared to the unloaded controls. CONCLUSIONS: A mechanical load of sufficient magnitude can inhibit ECM degradation by chondrocytes when stimulated by IL-1. The molecular mechanisms involved in this process are not clear but probably involve altered mechanochemical signal transduction between the ECM and chondrocyte. |
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Authors:
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P A Torzilli; M Bhargava; S Park; C T C Chen |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2009-09-01 |
Journal Detail:
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Title: Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society Volume: 18 ISSN: 1522-9653 ISO Abbreviation: Osteoarthr. Cartil. Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2010-02-03 Completed Date: 2010-05-11 Revised Date: 2011-07-19 |
Medline Journal Info:
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Nlm Unique ID: 9305697 Medline TA: Osteoarthritis Cartilage Country: England |
Other Details:
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Languages: eng Pagination: 97-105 Citation Subset: IM |
Copyright Information:
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Copyright 2009 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved. |
Affiliation:
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Laboratory for Soft Tissue Research, Tissue Engineering, Regeneration and Repair Program, Hospital for Special Surgery, New York, NY 10021-4010, USA. torzillip@hss.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aggrecans
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metabolism* Animals Antibodies, Monoclonal / immunology Cartilage, Articular / drug effects, enzymology, physiology* Cattle Collagen / metabolism* Endopeptidases / immunology, metabolism Interleukin-1 / pharmacology* Matrix Metalloproteinases / immunology, metabolism Stress, Mechanical |
| Grant Support | |
ID/Acronym/Agency:
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AR45748/AR/NIAMS NIH HHS; C06-RR12538-01/RR/NCRR NIH HHS; R01 AR045748-09/AR/NIAMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Aggrecans; 0/Antibodies, Monoclonal; 0/Interleukin-1; 9007-34-5/Collagen; EC 3.4.-/Endopeptidases; EC 3.4.24.-/Matrix Metalloproteinases; EC 3.4.99.-/aggrecanase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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