Document Detail

Mechanical interactions between ice crystals and red blood cells during directional solidification.
MedLine Citation:
PMID:  7988158     Owner:  NLM     Status:  MEDLINE    
Experiments in which red blood cells were frozen on a directional solidification stage under a microscope show that there is a mechanical interaction between ice crystals and cells in which cells are pushed and deformed by the ice crystals. The mechanical interaction occurs during freezing of cells in physiological saline and is significantly inhibited by the addition of 20% v/v glycerol to the solution. The addition of osmotically insignificant quantities of antifreeze proteins from the winter flounder or ocean pout to the physiological saline with 20% v/v glycerol generates strong mechanical interactions between the ice and the cells. The cells were destroyed during freezing in physiological saline, survived freezing in physiological saline with glycerol, and were completely destroyed by the addition of antifreeze proteins to the solution with glycerol. The difference in cell survival through freezing and thawing appears to be related, in part, to the habit of ice crystal growing in the suspension of red blood cells and the nature of mechanical interaction between the ice crystal and the cells. This suggests that mechanical damage may be a factor during cryopreservation of cells.
H Ishiguro; B Rubinsky
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cryobiology     Volume:  31     ISSN:  0011-2240     ISO Abbreviation:  Cryobiology     Publication Date:  1994 Oct 
Date Detail:
Created Date:  1995-01-12     Completed Date:  1995-01-12     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  483-500     Citation Subset:  IM    
Institute of Engineering Mechanics, University of Tsukuba, Japan.
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MeSH Terms
Antifreeze Proteins
Blood Preservation / adverse effects,  methods*
Cell Survival
Cryopreservation / methods*
Cryoprotective Agents
Erythrocytes* / drug effects,  ultrastructure
Evaluation Studies as Topic
Glycoproteins / pharmacology
Sodium Chloride
Reg. No./Substance:
0/Antifreeze Proteins; 0/Cryoprotective Agents; 0/Glycoproteins; 0/Ice; 56-81-5/Glycerol; 7647-14-5/Sodium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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