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Mechanical Properties of Tissue-Engineered Vascular Constructs Produced Using Arterial or Venous Cells.
MedLine Citation:
PMID:  21457095     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
There is a clinical need for better blood vessel substitutes since current surgical procedures are limited by the availability of suitable autologous vessels and suboptimal behavior of synthetic grafts in small caliber arterial graft (< 5 mm) applications. The aim of the present study was to compare the mechanical properties of arterial and venous tissue-engineered vascular constructs produced by the self-assembly approach using cells extracted from either the artery or vein harvested from the same human umbilical cord. The production of a vascular construct comprised of a media and an adventitia (TEVMA) was achieved by rolling a continuous tissue sheet containing both SMCs and adventitial fibroblasts grown contiguously in the same tissue culture plate. Histology and immunofluorescence staining were used to evaluate the structure and composition of the extracellular matrix (ECM) of the vascular constructs. The mechanical strength was assessed by uniaxial tensile testing, while viscoelastic behavior was evaluated by stepwise stress-relaxation and by cyclic loading hysteresis analysis. Tensile testing showed that the use of arterial cells resulted in stronger and stiffer constructs when compared to those produced using venous cells. Moreover, cyclic loading demonstrated that constructs produced using arterial cells were able to bear higher loads for the same amount of strain when compared to venous constructs. These results indicate that cells isolated from umbilical cord can be used to produce vascular constructs. Arterial constructs possessed superior mechanical properties when compared to venous constructs produced using cells isolated from the same human donor. This study highlights the fact that SMCs and fibroblasts originating from different cell sources can potentially lead to distinct tissue properties when used in tissue engineering applications.
Authors:
Robert Gauvin; Maxime D Guillemette; Todd Galbraith; Jean-Michel Bourget; Danielle Larouche; Hugo Marcoux; David Aubé; Cindy Hayward; Francois A Auger; Lucie Germain
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-4-2
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  -     ISSN:  1937-335X     ISO Abbreviation:  -     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-4-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Harvard-MIT, HST, 65 Landsdowne St, room 252, Cambridge, Massachusetts, United States, 02139, 404-277-4848; rgauvin@mit.edu.
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