Document Detail

Mechanical loading of stem cells for improvement of transplantation outcome in a model of acute myocardial infarction: the role of loading history.
MedLine Citation:
PMID:  22280442     Owner:  NLM     Status:  MEDLINE    
Stem cell therapy for tissue repair is a rapidly evolving field and the factors that dictate the physiological responsiveness of stem cells remain under intense investigation. In this study we hypothesized that the mechanical loading history of muscle-derived stem cells (MDSCs) would significantly impact MDSC survival, host tissue angiogenesis, and myocardial function after MDSC transplantation into acutely infarcted myocardium. Mice with acute myocardial infarction by permanent left coronary artery ligation were injected with either nonstimulated (NS) or mechanically stimulated (MS) MDSCs. Mechanical stimulation consisted of stretching the cells with equibiaxial stretch with a magnitude of 10% and frequency of 0.5 Hz. MS cell-transplanted hearts showed improved cardiac contractility, increased numbers of host CD31+ cells, and decreased fibrosis, in the peri-infarct region, compared to the hearts treated with NS MDSCs. MS MDSCs displayed higher vascular endothelial growth factor expression than NS cells in vitro. These findings highlight an important role for cyclic mechanical loading preconditioning of donor MDSCs in optimizing MDSC transplantation for myocardial repair.
Theresa R Cassino; Lauren Drowley; Masaho Okada; Sarah A Beckman; Bradley Keller; Kimimasa Tobita; Philip R Leduc; Johnny Huard
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-03-07
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  18     ISSN:  1937-335X     ISO Abbreviation:  Tissue Eng Part A     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-06-04     Completed Date:  2012-09-26     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1101-8     Citation Subset:  IM    
Department of Orthopaedic Surgery, Children's Hospital of Pittsburgh, Pittsburgh, Pennsylvania, USA.
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MeSH Terms
Cell Differentiation
Cell Proliferation
Cell Survival
Cicatrix / pathology,  physiopathology
Disease Models, Animal
Heart Function Tests
Ischemic Preconditioning, Myocardial
Mice, Inbred C57BL
Myocardial Infarction / pathology,  physiopathology,  therapy*
Myocardium / pathology
Neovascularization, Physiologic
Oxidative Stress
Stem Cell Transplantation*
Stem Cells / cytology*
Stress, Mechanical*
Treatment Outcome
Vascular Endothelial Growth Factor A / metabolism
Grant Support
Reg. No./Substance:
0/Vascular Endothelial Growth Factor A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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