Document Detail


Mechanical interactions of mouse mammary gland cells with collagen in a three-dimensional construct.
MedLine Citation:
PMID:  20411334     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An effort to understand the development of breast cancer motivates the study of mammary gland cells and their interactions with the extracellular matrix. A mixture of mammary gland epithelial cells (normal murine mammary gland), collagen, and fluorescent beads was loaded into microchannels and observed via four-dimensional imaging. Collagen concentrations of 1.3, 2, and 3 mg/mL were used. The displacements of the beads were used to calculate strains in the 3D matrix. To ensure physiologically relevant materials properties for analysis, the collagen was characterized using independent tensile testing with strain rates in the range of those measured in the cell-gel constructs. 3D elastic theory for an isotropic material was employed to calculate the stress. The technique presented adds to the field of measuring cell-generated stresses by providing the capability of measuring 3D stresses locally around a single cell and using physiologically relevant materials properties for analysis. The highest strains were observed in the most compliant matrix. Additionally, the stresses fluctuated over time due to the cells' interaction with the collagen matrix.
Authors:
M D C Lopez-Garcia; D J Beebe; W C Crone
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-22
Journal Detail:
Title:  Annals of biomedical engineering     Volume:  38     ISSN:  1573-9686     ISO Abbreviation:  Ann Biomed Eng     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-09     Completed Date:  2010-12-07     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  0361512     Medline TA:  Ann Biomed Eng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2485-98     Citation Subset:  IM    
Affiliation:
Material Science Program, Wisconsin Institutes for Medical Research, University of Wisconsin, Madison, WI, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biocompatible Materials / chemistry,  metabolism
Cell Line
Cells
Collagen* / chemistry,  metabolism
Epithelial Cells / cytology
Extracellular Matrix / chemistry,  metabolism
Female
Gels / chemistry,  metabolism
Mammary Glands, Animal / cytology*
Mechanical Phenomena
Mice
Tissue Engineering / methods
Tissue Scaffolds / chemistry*
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 0/Gels; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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