Document Detail

Mechanical interactions of mouse mammary gland cells with collagen in a three-dimensional construct.
MedLine Citation:
PMID:  20411334     Owner:  NLM     Status:  MEDLINE    
An effort to understand the development of breast cancer motivates the study of mammary gland cells and their interactions with the extracellular matrix. A mixture of mammary gland epithelial cells (normal murine mammary gland), collagen, and fluorescent beads was loaded into microchannels and observed via four-dimensional imaging. Collagen concentrations of 1.3, 2, and 3 mg/mL were used. The displacements of the beads were used to calculate strains in the 3D matrix. To ensure physiologically relevant materials properties for analysis, the collagen was characterized using independent tensile testing with strain rates in the range of those measured in the cell-gel constructs. 3D elastic theory for an isotropic material was employed to calculate the stress. The technique presented adds to the field of measuring cell-generated stresses by providing the capability of measuring 3D stresses locally around a single cell and using physiologically relevant materials properties for analysis. The highest strains were observed in the most compliant matrix. Additionally, the stresses fluctuated over time due to the cells' interaction with the collagen matrix.
M D C Lopez-Garcia; D J Beebe; W C Crone
Related Documents :
2257604 - Ontogenetic development of synovial a cells in fetal and neonatal rat knee joints.
16701834 - Rapid and complete cellularization of hydroxyapatite for bone tissue engineering.
16969754 - 'uromaix' scaffolds: novel collagen matrices for application in tissue engineering of t...
18694294 - Tailoring fiber diameter in electrospun poly(epsilon-caprolactone) scaffolds for optima...
8012034 - Combined effects of 1,25-dihydroxyvitamin d3 and tamoxifen on the growth of mcf-7 and z...
17942254 - Effect of surface properties of liposomes coated with a modified polyvinyl alcohol (pva...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-22
Journal Detail:
Title:  Annals of biomedical engineering     Volume:  38     ISSN:  1573-9686     ISO Abbreviation:  Ann Biomed Eng     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-09     Completed Date:  2010-12-07     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  0361512     Medline TA:  Ann Biomed Eng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2485-98     Citation Subset:  IM    
Material Science Program, Wisconsin Institutes for Medical Research, University of Wisconsin, Madison, WI, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Biocompatible Materials / chemistry,  metabolism
Cell Line
Collagen* / chemistry,  metabolism
Epithelial Cells / cytology
Extracellular Matrix / chemistry,  metabolism
Gels / chemistry,  metabolism
Mammary Glands, Animal / cytology*
Mechanical Phenomena
Tissue Engineering / methods
Tissue Scaffolds / chemistry*
Reg. No./Substance:
0/Biocompatible Materials; 0/Gels; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Emerging techniques in stratified designs and continuous gradients for tissue engineering of interfa...
Next Document:  IL-1beta induces ER stress in a JNK dependent manner that determines cell death in human pancreatic ...