| The Mec1p and Tel1p checkpoint kinases allow humanized yeast to tolerate chronic telomere dysfunctions by suppressing telomere fusions. | |
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MedLine Citation:
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PMID: 19007917 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this work we report that budding yeasts carrying human-type telomeric repeats at their chromosome termini show a chronic activation of the Rad53-dependent DNA damage checkpoint pathway and a G2/M cell cycle delay. Furthermore, in the absence of either TEL1/ATM or MEC1/ATR genes, which encodes phosphatidylinositol 3-kinase-related kinases (PIKKs), we detected telomere fusions, whose appearance correlates with a reduced cell viability and a high rate of genome instability. Based on sequence analysis, telomere fusions occurred primarily between ultrashort telomeres. Microcolony formation assays argue against the possibility that fusion-containing cells are eliminated by PIKK-dependent signalling. These findings reveal that humanized telomeres in yeast cells are sensed as a chronically damaged DNA but do not greatly impair cell viability as long as the cells have a functional DNA damage checkpoint. |
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Authors:
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Enea Gino di Domenico; Cristina Auriche; Valeria Viscardi; Maria Pia Longhese; Eric Gilson; Fiorentina Ascenzioni |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-11-28 |
Journal Detail:
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Title: DNA repair Volume: 8 ISSN: 1568-7864 ISO Abbreviation: DNA Repair (Amst.) Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2009-01-12 Completed Date: 2009-04-07 Revised Date: 2009-06-02 |
Medline Journal Info:
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Nlm Unique ID: 101139138 Medline TA: DNA Repair (Amst) Country: Netherlands |
Other Details:
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Languages: eng Pagination: 209-18 Citation Subset: IM |
Affiliation:
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Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Roma "La Sapienza", Roma, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Chromosomes, Fungal / metabolism DNA Damage DNA-Binding Proteins Genomic Instability Humans Intracellular Signaling Peptides and Proteins / metabolism* Microbial Viability Protein-Serine-Threonine Kinases / metabolism* Saccharomyces cerevisiae / cytology, enzymology* Saccharomyces cerevisiae Proteins / metabolism* Telomere / enzymology*, pathology* |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/Intracellular Signaling Peptides and Proteins; 0/LIF1 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; EC 2.7.11.1/MEC1 protein, S cerevisiae; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/TEL1 protein, S cerevisiae |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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