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Measuring transport and accumulation of radiolabeled substrates in oocytes and embryos.
MedLine Citation:
PMID:  23138951     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Radiolabeled compounds that are substrates for transmembrane transporters can be used to study transport and metabolism in mammalian oocytes and preimplantation embryos. Because even very small amounts of radioisotopes can be detected, these techniques are feasible to use with only a few oocytes or embryos, even down to the level of single oocytes or embryos. Here, we describe the methods for determining the transport and accumulation of radiolabeled compounds into oocytes and preimplantation embryos and the determination of the rate of saturable transport via specific transporters in the plasma membrane.
Authors:
Jay M Baltz; Hannah E Corbett; Samantha Richard
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  957     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  163-78     Citation Subset:  IM    
Affiliation:
Departments of Obstetrics & Gynecology and Cellular & Molecular Medicine, Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON, Canada, jmbaltz@ohri.ca.
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