| Measuring telomerase activity in various human tumors in routine histology and cytology. | |
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MedLine Citation:
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PMID: 14719139 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to investigate telomerase reactivation, to quantitatively measure the human telomerase reverse transcriptase (hTERT) content and telomerase activity level (TA) in routine histological and cytological samples, and to examine the relationship between these values and morphological factors. We analyzed 86 (35 cytological and 51 histological) lesions which were divided into four main groups: renal tumors, soft tissue tumors, bladder-urine and thyroid gland lesions. The relative expression of mRNA of hTERT was examined by real-time polymerase chain reaction (RT-PCR). Almost all of the renal cell carcinomas showed TA. In soft tissue tumors no correlation was seen between TA and histogenesis, aggressiveness and prognosis. Concerning cytological material a very good correlation was seen between TA and the benign or malignant nature of these tumors (92.3% specificity and 60% sensitivity). Our results indicated that TA can be used beside histology also in cytologic samples, for example in the preoperative differential diagnosis of the thyroid gland lesions and urine samples. Telomerase reactivation may not play an important role in tumorigenesis in STT. Useful observations can be made by concentrating not only on one group of diseases or localization but the unselected analysis of routine diagnostic cases can also be of help both in diagnosis and therapy. |
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Authors:
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Rita Beáta Kovács; Magdolna Bollmann; Norbert Speich; Reinhard Bollmann; Miklós Bodó; Zoltán Sápi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: International journal of molecular medicine Volume: 13 ISSN: 1107-3756 ISO Abbreviation: Int. J. Mol. Med. Publication Date: 2004 Feb |
Date Detail:
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Created Date: 2004-01-13 Completed Date: 2004-03-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9810955 Medline TA: Int J Mol Med Country: Greece |
Other Details:
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Languages: eng Pagination: 303-9 Citation Subset: IM |
Affiliation:
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First Department of Pathology and Experimental Cancer Research, Teaching Unit, Faculty of Medicine, Semmelweis University, Budapest, Hungary. ritabea@freemail.hu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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DNA, Complementary
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metabolism DNA-Binding Proteins Humans Neoplasms / enzymology* RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction Telomerase / biosynthesis, genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/DNA-Binding Proteins; 0/RNA, Messenger; EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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