Document Detail

Measuring passive myocardial stiffness in Drosophila melanogaster to investigate diastolic dysfunction.
MedLine Citation:
PMID:  22225769     Owner:  NLM     Status:  MEDLINE    
Aging is marked by a decline in LV diastolic function, which encompasses abnormalities in diastolic relaxation, chamber filling and/or passive myocardial stiffness. Genetic tractability and short life span make Drosophila melanogaster an ideal organism to study the effects of aging on heart function, including senescent-associated changes in gene expression and in passive myocardial stiffness. However, use of the Drosophila heart tube to probe deterioration of diastolic performance is subject to at least two challenges: the extent of genetic homology to mammals and the ability to resolve mechanical properties of the bilayered fly heart, which consists of a ventral muscle layer that covers the contractile cardiomyocytes. Here, we argue for widespread use of Drosophila as a novel myocardial aging model by (1) describing diastolic dysfunction in flies, (2) discussing how critical pathways involved in dysfunction are conserved across species and (3) demonstrating the advantage of an atomic force microscopy-based analysis method to measure stiffness of the multilayered Drosophila heart tube versus isolated myocytes from other model systems. By using powerful Drosophila genetic tools, we aim to efficiently alter changes observed in factors that contribute to diastolic dysfunction to understand how one might improve diastolic performance at advanced ages in humans.
Gaurav Kaushik; Alexander C Zambon; Alexander Fuhrmann; Sanford I Bernstein; Rolf Bodmer; Adam J Engler; Anthony Cammarato
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Journal of cellular and molecular medicine     Volume:  16     ISSN:  1582-4934     ISO Abbreviation:  J. Cell. Mol. Med.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-31     Completed Date:  2012-12-03     Revised Date:  2014-09-21    
Medline Journal Info:
Nlm Unique ID:  101083777     Medline TA:  J Cell Mol Med     Country:  England    
Other Details:
Languages:  eng     Pagination:  1656-62     Citation Subset:  IM    
Copyright Information:
© 2012 The Authors Journal of Cellular and Molecular Medicine © 2012 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd.
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MeSH Terms
Aging / pathology
Diastole / physiology*
Disease Models, Animal
Drosophila melanogaster / physiology*
Myocardium / pathology*
Grant Support
1P01-AG033561/AG/NIA NIH HHS; 1P01-HL098053/HL/NHLBI NIH HHS; 1R01-GM32443/GM/NIGMS NIH HHS; 1R01-HL085481/HL/NHLBI NIH HHS; 1R21-HL106529/HL/NHLBI NIH HHS; 1T32HL105373-01/HL/NHLBI NIH HHS; R01 GM032443/GM/NIGMS NIH HHS; R01 HL054732/HL/NHLBI NIH HHS; R21 HL106529/HL/NHLBI NIH HHS; R21 HL106529-01A1/HL/NHLBI NIH HHS

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