| Measuring the lifetime of singlet oxygen in a single cell: addressing the issue of cell viability. | |
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MedLine Citation:
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PMID: 17914485 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Singlet molecular oxygen, O(2)(a(1)Delta(g)), has been detected from single neurons and HeLa cells in time-resolved optical experiments by its 1270 nm phosphorescence (a(1)Delta(g)--> X(3)Sigma(-)(g)) upon irradiation of a photosensitizer incorporated into the cell. The cells were maintained in a buffered medium and their viability was assessed by live/dead assays. To facilitate the detection of singlet oxygen, intracellular H(2)O was replaced with D(2)O by an osmotic de- and rehydration process. The effect of this insult on the cells was likewise assessed. The data indicate that, in the complicated transition from a "live" to "dead" cell, the majority of our cells have the metabolic activity and morphology characteristic of a live cell. Quenching experiments demonstrate that the singlet oxygen lifetime in our cells is principally determined by interactions with intracellular water and not by interactions with other cell constituents. The data indicate that in a viable, metabolically-functioning, and H(2)O-containing cell, the lifetime of singlet oxygen is approximately 3 micros. This is consistent with our previous reports, and confirms that the singlet oxygen lifetime in a cell is much longer than hitherto believed. This implies that, in a cell, singlet oxygen is best characterized as a selective rather than reactive intermediate. This is important when considering roles played by singlet oxygen as a signaling agent and as a component in events that result in cell death. The data reported herein also demonstrate that spatially-resolved optical probes can be used to monitor selected events in the light-induced, singlet-oxygen-mediated death of a single cell. |
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Authors:
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Sonja Hatz; John D C Lambert; Peter R Ogilby |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-07-30 |
Journal Detail:
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Title: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology Volume: 6 ISSN: 1474-905X ISO Abbreviation: Photochem. Photobiol. Sci. Publication Date: 2007 Oct |
Date Detail:
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Created Date: 2007-10-04 Completed Date: 2008-01-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101124451 Medline TA: Photochem Photobiol Sci Country: England |
Other Details:
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Languages: eng Pagination: 1106-16 Citation Subset: IM |
Affiliation:
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Department of Chemistry University of Aarhus, DK-8000, Arhus, Denmark. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis Cell Survival HEPES / chemistry Hela Cells Humans Neurons / chemistry, metabolism* Photosensitizing Agents / metabolism* Rats Singlet Oxygen / analysis, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Photosensitizing Agents; 17778-80-2/Singlet Oxygen; 7365-45-9/HEPES |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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