Document Detail

Measuring the elastic properties of living cells with atomic force microscopy indentation.
MedLine Citation:
PMID:  23027009     Owner:  NLM     Status:  In-Data-Review    
Atomic force microscopy (AFM) is a powerful and versatile tool for probing the mechanical properties of biological samples. This chapter describes the procedures for using AFM indentation to measure the elastic moduli of living cells. We include step-by-step instructions for cantilever calibration and data acquisition using a combined AFM/optical microscope system, as well as a detailed protocol for data analysis. Our protocol is written specifically for the BioScope™ Catalyst™ AFM system (Bruker AXS Inc.); however, most of the general concepts can be readily translated to other commercial systems.
Joanna L Mackay; Sanjay Kumar
Related Documents :
23767719 - Ground-state cooling of a single atom at the center of an optical cavity.
22995789 - Exploring atomic-scale lateral forces in the attractive regime: a case study on graphit...
21900069 - Vesicoureteral reflux in children: a phantom study of microwave heating and radiometric...
17904729 - Modelling metapopulations with stochastic membrane systems.
24559339 - Computational efficiency improvement with wigner rotation technique in studying atoms i...
7796269 - Simulating efficiently the evolution of dna sequences.
16194299 - The danger of wishing for chaos.
18073899 - High-precision frequency-domain measurements of the optical properties of turbid media.
18250709 - High-power and high-gain organic dye-doped polymer optical fiber amplifiers: novel tech...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  931     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-10-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  313-29     Citation Subset:  IM    
Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Mica functionalization for imaging of DNA and protein-DNA complexes with atomic force microscopy.
Next Document:  Atomic force microscopy functional imaging on vascular endothelial cells.