Document Detail


Measuring plasma fatty acid oxidation with intravenous bolus injection of 3H- and 14C-fatty acid.
MedLine Citation:
PMID:  23093549     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Accurate measures of plasma FA oxidation can improve our understanding of diseases characterized by impaired FA oxidation. We describe and compare the 24 h time-courses of FA oxidation using bolus injections of [1-(14)C]palmitate versus [9,10-(3)H]palmitate under postabsorptive, postprandial, and walking conditions. Fifty-one men and 95 premenopausal women participated in one condition (postabsorptive, postprandial, or walking), one tracer ((14)C- or (3)H-labeled), and an acetate or palmitate study. Groups were matched for sex, age, and body mass index (BMI). At 24 h, cumulative [(3)H]acetate recovery as (3)H(2)O was 80 ± 6%, 78 ± 2%, and 81 ± 6% in the postabsorptive, postprandial, and walking conditions, respectively (not significant). Model-predicted maximum [1-(14)C]acetate recovery as expired (14)CO(2) was 59 ± 12%, 52 ± 8%, and 65 ± 10% in the postabsorptive, postprandial, and walking condition, respectively (one way ANOVA, P = 0.12). When corrected with the corresponding acetate recovery factors, 24 h time-courses of FFA oxidation were similar between [1-(14)C]palmitate and [9,10-(3)H]palmitate in all three conditions. In contrast to previous meal ingestion studies, an acetate-hydrogen recovery factor was needed to achieve comparable oxidation rates using an intravenous bolus of [(3)H]palmitate. In conclusion, intravenous boluses of [9,10-(3)H]palmitate versus [1-(14)C]palmitate gave similar estimates of 24 h cumulative FFA oxidation in age-, sex- and BMI-matched individuals.
Authors:
Christina Koutsari; Asem H Ali; Manpreet S Mundi; Michael D Jensen
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Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-10-23
Journal Detail:
Title:  Journal of lipid research     Volume:  54     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-17     Completed Date:  2013-05-28     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  254-64     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Absorption
Acetates / administration & dosage,  blood*,  chemistry,  metabolism*
Adult
Blood Chemical Analysis / methods*
Carbon Radioisotopes / chemistry
Female
Humans
Injections, Intravenous
Male
Oxidation-Reduction
Palmitates / administration & dosage,  blood*,  chemistry,  metabolism*
Postprandial Period
Radiochemistry
Time Factors
Tritium / chemistry*
Walking
Grant Support
ID/Acronym/Agency:
1 UL1 RR-024150-01/RR/NCRR NIH HHS; DK-40484/DK/NIDDK NIH HHS; DK-45343/DK/NIDDK NIH HHS; DK-50456/DK/NIDDK NIH HHS; R-00585//PHS HHS; UL1 RR024150/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Acetates; 0/Carbon Radioisotopes; 0/Palmitates; 10028-17-8/Tritium
Comments/Corrections

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