| Measuring Plasma Fatty Acid Oxidation with Intravenous Bolus Injection of 3H- and 14C-Fatty Acid. | |
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MedLine Citation:
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PMID: 23093549 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Accurate measures of plasma fatty acid oxidation can improve our understanding of diseases characterized by impaired fatty acid oxidation. We describe and compare the 24-hour time-courses of fatty acid oxidation using bolus injections of [1-(14)C]palmitate vs. [9,10-(3)H]palmitate under postabsorptive, postprandial, and walking conditions. Fifty one men and 95 premenopausal women participated in one condition (postabsorptive, postprandial, or walking), one tracer ((14)C- or (3)H-labeled), and an acetate or palmitate study. Groups were matched for sex, age, and BMI. At 24 hours, cumulative [(3)H]acetate recovery as (3)H(2)O was 80%±6%, 78%±2%, and 81%±6% in the postabsorptive, postprandial, and walking conditions, respectively (NS). Model-predicted maximum [1-(14)C]acetate recovery as expired (14)CO(2) was 59%±12%, 52%±8%, and 65%±10% in the postabsorptive, postprandial, and walking condition, respectively (one way ANOVA P=0.12). When corrected with the corresponding acetate recovery factors, 24-hour time-courses of free fatty acid (FFA) oxidation were similar between [1-(14)C]palmitate and [9,10-(3)H]palmitate in all three conditions. In contrast to previous meal ingestion studies, an acetate-hydrogen recovery factor was needed to achieve comparable oxidation rates using an intravenous bolus of [(3)H]palmitate. In conclusion, intravenous boluses of [9,10-(3)H]palmitate vs. [1-(14)C]palmitate gave similar estimates of 24-hour cumulative FFA oxidation in age-, sex- and BMI-matched individuals. |
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Authors:
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Christina Koutsari; Asem H Ali; Manpreet S Mundi; Michael D Jensen |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-10-23 |
Journal Detail:
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Title: Journal of lipid research Volume: - ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-10-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Mayo Clinic, United States. |
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