Document Detail


Measuring markers of liver function using a micropatterned paper device designed for blood from a fingerstick.
MedLine Citation:
PMID:  22390675     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This paper describes a paper-based microfluidic device that measures two enzymatic markers of liver function (alkaline phosphatase, ALP, and aspartate aminotransferase, AST) and total serum protein. A device consists of four components: (i) a top plastic sheet, (ii) a filter membrane, (iii) a patterned paper chip containing the reagents necessary for analysis, and (iv) a bottom plastic sheet. The device performs both the sample preparation (separating blood plasma from erythrocytes) and the assays; it also enables both qualitative and quantitative analysis of data. The data obtained from the paper-microfluidic devices show standard deviations in calibration runs and "spiked" standards that are acceptable for routine clinical use. This device illustrates a type of test useable for a range of assays in resource-poor settings.
Authors:
Sarah J Vella; Patrick Beattie; Rebecca Cademartiri; Anna Laromaine; Andres W Martinez; Scott T Phillips; Katherine A Mirica; George M Whitesides
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-03-02
Journal Detail:
Title:  Analytical chemistry     Volume:  84     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-03-21     Completed Date:  2012-07-23     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2883-91     Citation Subset:  IM    
Affiliation:
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, United States.
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MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase / metabolism
Aspartate Aminotransferases / metabolism
Blood Proteins / analysis*
Calibration
Equipment Design
Filtration / instrumentation
Humans
Liver / enzymology*
Liver Function Tests / instrumentation*
Microfluidic Analytical Techniques / instrumentation*
Paper*
Sensitivity and Specificity
Grant Support
ID/Acronym/Agency:
F32 GM078782-01A1/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Blood Proteins; EC 2.6.1.1/Aspartate Aminotransferases; EC 3.1.3.1/Alkaline Phosphatase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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