| Measuring D-serine efflux from mouse cortical brain slices using online microdialysis-capillary electrophoresis. | |
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MedLine Citation:
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PMID: 16703626 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Efflux of a number of important neurochemicals, including D-serine, L-serine, taurine, glutamate, and gamma-aminobutyric acid (GABA), from mouse cortical brain slices housed in a 7 microL perfusion chamber was monitored using online microdialysis-CE (MD-CE). Analyte concentrations could be measured every 20-27 s using the MD-CE instrument. Stimulation with high potassium induced increased release of D-serine. Kainic acid (KA) induced D-serine release, but this release was not blocked by 6-cyano-7-nitroquinoxaline-2,3-dione, suggesting that alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid/KA receptors do not mediate D-serine release. Application of L-serine, the precursor of D-serine, resulted in increased extracellular D-serine concentrations. L-Cysteine also increased extracellular D-serine levels in a partially Na+-dependent manner. The observed effects upon application of L-serine and L-cysteine support the involvement of ASC neutral amino acid transporters in regulating the extracellular concentration of D-serine concentration through competitive inhibition of uptake or increased release through heteroexchange. |
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Authors:
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Kylie B O'Brien; Michael T Bowser |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Electrophoresis Volume: 27 ISSN: 0173-0835 ISO Abbreviation: Electrophoresis Publication Date: 2006 May |
Date Detail:
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Created Date: 2006-05-24 Completed Date: 2006-08-11 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8204476 Medline TA: Electrophoresis Country: Germany |
Other Details:
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Languages: eng Pagination: 1949-56 Citation Subset: IM |
Affiliation:
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Department of Chemistry, University of Minnesota, Minneapolis, MN 55455, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain Chemistry Cerebral Cortex / drug effects, metabolism* Electrophoresis, Capillary / methods* Kainic Acid / pharmacology Kinetics Male Mice Mice, Inbred C57BL Microdialysis / methods* Online Systems Perfusion Potassium / pharmacology Receptors, AMPA / drug effects, metabolism Receptors, Kainic Acid / drug effects, metabolism Serine / analysis, chemistry, metabolism* Stereoisomerism |
| Grant Support | |
ID/Acronym/Agency:
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NS043304/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Receptors, AMPA; 0/Receptors, Kainic Acid; 487-79-6/Kainic Acid; 56-45-1/Serine; 7440-09-7/Potassium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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