Document Detail


Measurements of transposition frequency of insertion sequence IS1 by GFP hop-on assay.
MedLine Citation:
PMID:  20647675     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transposons play a significant role in the evolution of bacterial genomes. Quantifying frequency of transpositional events caused by a transposon will facilitate understanding its role. Here, we report successful measurement of the frequency of IS1 transposition using "GFP hop-on assay" in which transposition-dependent GFP expression is monitored by FACS. This assay allows easy assessment of IS transposition into the chromosomal DNA on a single-cell scale; this is an advantage over other conventional methods to measure transposition frequency.
Authors:
Takashi Saito; Taku Chibazakura; Kiwamu Takahashi; Hirofumi Yoshikawa; Yasuhiko Sekine
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of general and applied microbiology     Volume:  56     ISSN:  0022-1260     ISO Abbreviation:  J. Gen. Appl. Microbiol.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-07-21     Completed Date:  2010-08-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0165543     Medline TA:  J Gen Appl Microbiol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  187-92     Citation Subset:  IM    
Affiliation:
Department of Life Science, College of Science, Rikkyo (St. Paul's) University, Toshima-ku, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Cell Separation
DNA Transposable Elements*
Escherichia coli / genetics*
Flow Cytometry
Green Fluorescent Proteins / genetics*
Chemical
Reg. No./Substance:
0/DNA Transposable Elements; 147336-22-9/Green Fluorescent Proteins

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