Document Detail

Measurements of phagocytosis and phagosomal maturation.
MedLine Citation:
PMID:  18228442     Owner:  NLM     Status:  MEDLINE    
Phagocytosis is an essential component of the innate immune response. Invading microorganisms are engulfed by neutrophils and macrophages, and subsequently killed by a complex series of reactions that require fusion of the phagocytic vacuole with multiple endomembrane organelles. This vacuolar remodeling process, known as phagosomal maturation, is often the target of intracellular parasites that evade killing by the host immune system. This unit describes detailed protocols for the assessment of phagosome formation and maturation. Particular attention is given to Fc- and complement-receptor mediated phagocytosis, but the protocols provided can be readily adapted to other types of phagocytic processes.
Chung-Wai Chow; Gregory P Downey; Sergio Grinstein
Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.]     Volume:  Chapter 15     ISSN:  1934-2616     ISO Abbreviation:  Curr Protoc Cell Biol     Publication Date:  2004 May 
Date Detail:
Created Date:  2008-01-29     Completed Date:  2008-03-12     Revised Date:  2009-12-11    
Medline Journal Info:
Nlm Unique ID:  101287856     Medline TA:  Curr Protoc Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit 15.7     Citation Subset:  IM    
University of Toronto and Toronto General Hospital, Toronto, Canada.
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MeSH Terms
Antigens, Surface / metabolism
Cells, Cultured
Complement System Proteins / metabolism,  physiology
Erythrocytes / metabolism,  physiology
Flow Cytometry
Hydrogen-Ion Concentration
Laboratory Techniques and Procedures*
Models, Biological
Phagocytosis / physiology*
Phagosomes / metabolism*
Protein Binding
Receptors, IgG / physiology
Reg. No./Substance:
0/Antigens, Surface; 0/Receptors, IgG; 9007-36-7/Complement System Proteins

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