| Measurements of phagocytosis and phagosomal maturation. | |
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MedLine Citation:
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PMID: 18228442 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Phagocytosis is an essential component of the innate immune response. Invading microorganisms are engulfed by neutrophils and macrophages, and subsequently killed by a complex series of reactions that require fusion of the phagocytic vacuole with multiple endomembrane organelles. This vacuolar remodeling process, known as phagosomal maturation, is often the target of intracellular parasites that evade killing by the host immune system. This unit describes detailed protocols for the assessment of phagosome formation and maturation. Particular attention is given to Fc- and complement-receptor mediated phagocytosis, but the protocols provided can be readily adapted to other types of phagocytic processes. |
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Authors:
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Chung-Wai Chow; Gregory P Downey; Sergio Grinstein |
Publication Detail:
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Type: Journal Article; Review |
Journal Detail:
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Title: Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.] Volume: Chapter 15 ISSN: 1934-2616 ISO Abbreviation: Curr Protoc Cell Biol Publication Date: 2004 May |
Date Detail:
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Created Date: 2008-01-29 Completed Date: 2008-03-12 Revised Date: 2009-12-11 |
Medline Journal Info:
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Nlm Unique ID: 101287856 Medline TA: Curr Protoc Cell Biol Country: United States |
Other Details:
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Languages: eng Pagination: Unit 15.7 Citation Subset: IM |
Affiliation:
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University of Toronto and Toronto General Hospital, Toronto, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, Surface / metabolism Cells, Cultured Complement System Proteins / metabolism, physiology Erythrocytes / metabolism, physiology Flow Cytometry Humans Hydrogen-Ion Concentration Laboratory Techniques and Procedures* Microspheres Models, Biological Phagocytosis / physiology* Phagosomes / metabolism* Protein Binding Receptors, IgG / physiology |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Surface; 0/Receptors, IgG; 9007-36-7/Complement System Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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