Document Detail

Measurements of eosinophil activation before and after food challenges in adults with food hypersensitivity.
MedLine Citation:
PMID:  16757922     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Objective assessment of inflammatory reactions in the gastrointestinal tract could be useful in the diagnosis of food hypersensitivity. The aim of the present study was to investigate the involvement of eosinophils and mast cells in the inflammatory response of patients with food hypersensitivity before and after food challenges. METHODS: Eleven patients (4 with IgE-mediated allergy and 7 without) with food hypersensitivity and positive double-blind, placebo-controlled food challenge were subjected to food challenge in a single-blinded fashion. Four subjects with no known food hypersensitivity were recruited as controls. Placebo was given after a 1-week washout period followed by an active dose. Stool, urinary and serum samples were collected and symptoms were recorded in a diary. Fecal samples were analyzed for eosinophil protein X (F-EPX) and tryptase; urinary samples for EPX (U-EPX) and leukotriene E4 (U-LTE4) and serum samples were analyzed for eotaxin and food-specific IgE antibodies. RESULTS: Patients with IgE-mediated food allergy had increased levels of F-EPX compared to controls and tended to have lower serum levels of eotaxin compared to non-allergic patients and controls. U-LTE4 was significantly higher in allergic patients compared to non-allergic patients after challenge. Moreover, F-EPX correlated to U-LTE4 (p = 0.011). Reported symptoms, abdominal pain, distension, flatulence and nausea were similar in the allergic and non-allergic patients. CONCLUSION: The results strongly indicate that eosinophils are activated in the gastrointestinal tract of food-allergic patients but not in patients with non-allergic food hypersensitivity. Due to the inconsistent pattern of symptoms after placebo and active food challenge, it was not possible to relate the levels of inflammation markers to the recorded symptoms.
J van Odijk; C G B Peterson; S Ahlstedt; U Bengtsson; M P Borres; L Hulthén; J Magnusson; T Hansson
Publication Detail:
Type:  Controlled Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-06-02
Journal Detail:
Title:  International archives of allergy and immunology     Volume:  140     ISSN:  1018-2438     ISO Abbreviation:  Int. Arch. Allergy Immunol.     Publication Date:  2006  
Date Detail:
Created Date:  2006-07-19     Completed Date:  2006-09-05     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9211652     Medline TA:  Int Arch Allergy Immunol     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  334-41     Citation Subset:  IM    
Copyright Information:
Copyright 2006 S. Karger AG, Basel.
Department of Clinical Nutrition, Faculty of Medicine, Sahlgrenska Academy at Goteborg University, Gothenburg, Sweden.
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MeSH Terms
Abdominal Pain / etiology,  immunology
Chemokine CCL11
Chemokines, CC / blood
Double-Blind Method
Eating / immunology*
Eosinophil-Derived Neurotoxin / blood,  urine
Eosinophils / immunology*
Feces / chemistry
Food / adverse effects
Food Hypersensitivity / blood,  immunology*,  urine
Gastrointestinal Tract / chemistry,  immunology
Leukotriene E4 / urine
Middle Aged
Single-Blind Method
Time Factors
Reg. No./Substance:
0/CCL11 protein, human; 0/Chemokine CCL11; 0/Chemokines, CC; 75715-89-8/Leukotriene E4; EC 3.1.-/Eosinophil-Derived Neurotoxin

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