| Measurement of type I interferon production. | |
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MedLine Citation:
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PMID: 21400681 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The Basic Protocol in this unit describes measurement of murine interferon (IFN)α/β by intracellular staining for these cytokines and detection by flow cytometry. Alternate protocols detail an enzyme-linked immunoabsorbent assay (ELISA) for IFNα and a biological assay to measure IFN. The FACS assay allows measurement of IFNα/β production by defined cell populations, while ELISA measures secreted IFNα. The bioassay measures functional antiviral activity and is the most sensitive of the assays discussed. These assays therefore provide complementary methods to assess IFN production by murine cells. Curr. Protoc. Immunol. 92:14.21.1-14.21.11. © 2011 by John Wiley & Sons, Inc. |
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Authors:
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Rosalind E Seeds; Joanna L Miller |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Current protocols in immunology / edited by John E. Coligan ... [et al.] Volume: Chapter 14 ISSN: 1934-368X ISO Abbreviation: Curr Protoc Immunol Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-03-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9101651 Medline TA: Curr Protoc Immunol Country: United States |
Other Details:
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Languages: eng Pagination: Unit14.21 Citation Subset: IM |
Affiliation:
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Sir William Dunn School of Pathology, University of Oxford, United Kingdom. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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