Document Detail


Measurement of tissue acyl-CoAs using flow-injection tandem mass spectrometry: acyl-CoA profiles in short-chain fatty acid oxidation defects.
MedLine Citation:
PMID:  23117082     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The primary accumulating metabolites in fatty acid oxidation defects are intramitochondrial acyl-CoAs. Typically, secondary metabolites such as acylcarnitines, acylglycines and dicarboxylic acids are measured to study these disorders. Methods have not been adapted for tissue acyl-CoA measurement in defects with primarily acyl-CoA accumulation. Our objective was to develop a method to measure fatty acyl-CoA species that are present in tissues of mice with fatty acid oxidation defects using flow-injection tandem mass spectrometry. Following the addition of internal standards of [(13)C(2)] acetyl-CoA, [(13)C(8)] octanoyl-CoA, and [C(17)] heptadecanoic CoA, acyl-CoA's are extracted from tissue samples and are injected directly into the mass spectrometer. Data is acquired using a 506.9 neutral loss scan and multiple reaction-monitoring (MRM). This method can identify all long, medium and short-chain acyl-CoA species in wild type mouse liver including predicted 3-hydroxyacyl-CoA species. We validated the method using liver of the short-chain-acyl-CoA dehydrogenase (SCAD) knock-out mice. As expected, there is a significant increase in [C(4)] butyryl-CoA species in the SCAD -/- mouse liver compared to wild type. We then tested the assay in liver from the short-chain 3-hydroxyacyl-CoA dehydrogenase (SCHAD) deficient mice to determine the profile of acyl-CoA accumulation in this less predictable model. There was more modest accumulation of medium chain species including 3-hydroxyacyl-CoA's consistent with the known chain-length specificity of the SCHAD enzyme.
Authors:
Andrew A Palladino; Jie Chen; Staci Kallish; Charles A Stanley; Michael J Bennett
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-18
Journal Detail:
Title:  Molecular genetics and metabolism     Volume:  107     ISSN:  1096-7206     ISO Abbreviation:  Mol. Genet. Metab.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-21     Completed Date:  2013-04-25     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  9805456     Medline TA:  Mol Genet Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  679-83     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
Affiliation:
Division of Endocrinology, Department of Pediatrics, Children's Hospital of Philadelphia and Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
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MeSH Terms
Descriptor/Qualifier:
Acyl Coenzyme A / analysis*,  chemistry,  metabolism
Acyl-CoA Dehydrogenases / genetics,  metabolism
Animals
Fatty Acids, Volatile / chemistry,  metabolism
Liver / chemistry,  enzymology
Mice
Mice, Knockout
Oxidation-Reduction
Reproducibility of Results
Tandem Mass Spectrometry*
Grant Support
ID/Acronym/Agency:
R37 DK056268/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Acyl Coenzyme A; 0/Fatty Acids, Volatile; EC 1.3.-/Acyl-CoA Dehydrogenases
Comments/Corrections

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