Document Detail


Measurement of thermally produced volatile alkanes: an assay for plant hydroperoxy fatty acid evaluation.
MedLine Citation:
PMID:  7733454     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new method designed to monitor lipid peroxidation in plants has been set up with soybean hypocotyl/radicles. The hydroperoxy fatty acids present in situ are converted by rapid thermal treatment (80 s and 210 J g-1) of the biological sample into ethane and n-pentane, which are analyzed by gas chromatography. The method has been directly calibrated by quantification of the hydroperoxy fatty acids by silica-phase HPLC analysis of their reduced hydroxy derivatives. Hypocotyl/radicles from the two soybean cultivars Argenta and Soriano were submitted to various chemical oxidative treatments and were analyzed for both thermally produced volatile alkanes and hydroperoxy fatty acid levels. Our results showed that ethane and n-pentane production are in both cases closely correlated with linolenic as well as linoleic acid hydroperoxide levels (P < 0.001). Within a given plant material, thermal conversion of both hydroperoxides into alkanes occurred with yields which were not dependent on the oxidative treatment. These yields are however functions of the biological material since in Soriano and Argenta cultivars they were around 6 and 25%, respectively. Taking into account the last point, the alkane test cannot be used to directly quantify the absolute lipid hydroperoxide levels of plant tissues but it is convenient to monitor the peroxidative phenomenon as it occurs. The assay is easy and rapid to perform (analysis of 50 samples per day) since no sample preparation is needed, and the low detection limit (20 pmol of alkane g-1) permits the analysis of small samples.
Authors:
N Degousée; C Triantaphylidès; S Starek; G Iacazio; D Martini; C Bladier; R Voisine; J L Montillet
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  224     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1995 Jan 
Date Detail:
Created Date:  1995-05-30     Completed Date:  1995-05-30     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  524-31     Citation Subset:  IM    
Affiliation:
Centre d'Etudes de Cadarache, Département de Physiologie Végétale et Ecosystèmes, Saint Paul lez Durance, Cedex, France.
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MeSH Terms
Descriptor/Qualifier:
Alkanes / analysis*,  metabolism
Chromatography, High Pressure Liquid
Heating
Linoleic Acid
Linoleic Acids / analysis*,  metabolism
Lipid Peroxidation
Lipid Peroxides / analysis*,  metabolism
Pentanes / analysis,  metabolism
Plants / chemistry*,  metabolism
Seeds / chemistry,  metabolism
Soybeans / chemistry,  metabolism
alpha-Linolenic Acid / analysis*,  metabolism
Chemical
Reg. No./Substance:
0/Alkanes; 0/Linoleic Acids; 0/Lipid Peroxides; 0/Pentanes; 109-66-0/pentane; 2197-37-7/Linoleic Acid; 23017-93-8/13-hydroperoxy-9,11-octadecadienoic acid; 463-40-1/alpha-Linolenic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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