Document Detail


Measurement of testosterone in infant fecal samples.
MedLine Citation:
PMID:  21774027     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: This study reports the validation of a noninvasive method for repeated assessment of testosterone from infant fecal samples.
METHODS: Fecal samples were collected from cotton diaper liners, subjected to methanol extraction, and assayed using a modified commercial testosterone RIA kit.
RESULTS: Method validity was supported by a recovery near 100%, a sensitivity of 1.23 pg/ml, and inter- and intra-assay coefficients of variations less than 10 and 15%, respectively. Testosterone was detected in all samples from male and female infants aged 2 weeks to 15 months.
CONCLUSIONS: Fecal assessment is supported as a novel, non-invasive tool for studying testosterone during early human development.
Authors:
Amanda L Thompson; Patricia L Whitten; Michelle Lampl
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Validation Studies     Date:  2011-07-19
Journal Detail:
Title:  American journal of human biology : the official journal of the Human Biology Council     Volume:  23     ISSN:  1520-6300     ISO Abbreviation:  Am. J. Hum. Biol.     Publication Date:    2011 Nov-Dec
Date Detail:
Created Date:  2011-10-11     Completed Date:  2012-02-06     Revised Date:  2012-10-09    
Medline Journal Info:
Nlm Unique ID:  8915029     Medline TA:  Am J Hum Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  820-2     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Wiley-Liss, Inc.
Affiliation:
Department of Anthropology, University of North Carolina at Chapel Hill, USA. althomps@email.unc.edu
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MeSH Terms
Descriptor/Qualifier:
Feces / chemistry*
Female
Humans
Infant
Infant, Newborn
Male
Radioimmunoassay / instrumentation,  methods*
Reproducibility of Results
Sensitivity and Specificity
Testosterone / analysis*
United States
Grant Support
ID/Acronym/Agency:
R24 HD050924/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
58-22-0/Testosterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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