Document Detail

Measurement of sulfur-containing compounds involved in the metabolism and transport of cysteamine and cystamine. Regional differences in cerebral metabolism.
MedLine Citation:
PMID:  19523884     Owner:  NLM     Status:  MEDLINE    
An HPLC method with coulometric detection is presented for the quantitation of cysteamine, cystamine, thialysine, glutathione, glutathione disulfide and an oxidized metabolite of thialysine [S-(2-aminoethyl)-L-cysteine ketimine decarboxylated dimer (AECK-DD)]. The advantage of coulometric detection is that derivatization is unnecessary if the analyte is redox sensitive. The method was used to quantitate several sulfur-containing compounds in plasma and brain following gavage feeding of cysteamine to rats. Cysteamine, cystamine, thialysine and AECK-DD were detected in the brains of these animals. Interestingly, cysteamine treatment resulted in greatly elevated levels of cerebral methionine, despite the fact that cysteamine is not a precursor of methionine.
John T Pinto; Tetyana Khomenko; Sandor Szabo; Gordon D McLaren; Travis T Denton; Boris F Krasnikov; Thomas M Jeitner; Arthur J L Cooper
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-05-28
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  877     ISSN:  1873-376X     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-09-23     Completed Date:  2009-11-25     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  3434-41     Citation Subset:  IM    
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA.
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MeSH Terms
Biological Transport
Cerebrum / chemistry,  metabolism*
Chromatography, High Pressure Liquid / instrumentation,  methods*
Cystamine / metabolism*
Cysteamine / metabolism*
Rats, Sprague-Dawley
Sulfur Compounds / analysis*,  blood,  metabolism
Grant Support
2P01 AG14930/AG/NIA NIH HHS; CA111842/CA/NCI NIH HHS; P01 AG014930-05A20004/AG/NIA NIH HHS
Reg. No./Substance:
0/Sulfur Compounds; 51-85-4/Cystamine; 60-23-1/Cysteamine

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