Document Detail

Measurement of steroidogenesis in rodent Leydig cells: a comparison between pregnenolone and testosterone production.
MedLine Citation:
PMID:  2789157     Owner:  NLM     Status:  MEDLINE    
The efficiency and specificity of inhibition of pregnenolone metabolism in mature, immature rat Leydig cells, mouse and tumour Leydig cells by SU-10603, a 17 alpha-hydroxylase inhibitor and epostane (WIN-32729), a 3 beta-hydroxysteroid dehydrogenase inhibitor, were studied. Metabolism of [14C]pregnenolone by mature rat Leydig cells was inhibited for more than 95% in the presence of 20 microM SU-10603 and 5 microM epostane. The sum of the different steroids produced by Leydig cells from immature rats incubated in the presence of a 5 alpha-reductase inhibitor was only 80% of pregnenolone production in the presence of SU-10603 and epostane. Pregnenolone metabolism could also be inhibited in tumour Leydig cells but not in mouse Leydig cells. Pregnenolone and testosterone production by Leydig cells from mature rats were similar when steroidogenesis is maximally stimulated by luteinizing hormone (LH). However, in the presence of LH and bovine serum albumin (bSA), or 22 R-hydroxycholesterol and bSA, pregnenolone production was 1.7- and 6-fold higher respectively, than testosterone production. The data show that for measuring the steroidogenic activity of Leydig cells estimation of pregnenolone production is more reliable than measuring testosterone production. At high activities of the cholesterol side-chain cleavage (CSCC) the conversion of pregnenolone into testosterone may become the rate-limiting step for testosterone production. Under all conditions the conversion of cholesterol into pregnenolone is the (hormonal regulated) rate-determining step for steroidogenesis.
L van Haren; J Cailleau; F F Rommerts
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article    
Journal Detail:
Title:  Molecular and cellular endocrinology     Volume:  65     ISSN:  0303-7207     ISO Abbreviation:  Mol. Cell. Endocrinol.     Publication Date:  1989 Aug 
Date Detail:
Created Date:  1989-10-23     Completed Date:  1989-10-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7500844     Medline TA:  Mol Cell Endocrinol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  157-64     Citation Subset:  IM    
Department of Biochemistry II, Erasmus University Rotterdam, The Netherlands.
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MeSH Terms
3-Hydroxysteroid Dehydrogenases / antagonists & inhibitors
Androstenols / pharmacology
Leydig Cells / metabolism*
Luteinizing Hormone / physiology
Pregnenolone / biosynthesis*
Rats, Inbred Strains
Steroid 17-alpha-Hydroxylase / antagonists & inhibitors
Testosterone / biosynthesis*
Tetrahydronaphthalenes / pharmacology
Reg. No./Substance:
0/Androstenols; 0/Tetrahydronaphthalenes; 145-13-1/Pregnenolone; 58-22-0/Testosterone; 786-97-0/SU 10603; 80471-63-2/epostane; 9002-67-9/Luteinizing Hormone; EC 1.1.-/3-Hydroxysteroid Dehydrogenases; EC 17-alpha-Hydroxylase

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