Document Detail

Measurement of stain on extracted teeth using spectrophotometry and digital image analysis.
MedLine Citation:
PMID:  17615027     Owner:  NLM     Status:  MEDLINE    
AIM: The aim of this study was to assess the reliability and validate a customized image analysis system, designed for use within clinical trials of general dental hygiene and whitening products, for the measurement of stain levels on extracted teeth and to compare it with reflectance spectrophotometry. METHOD: Twenty non-carious extracted teeth were soaked in an artificial saliva, brushed for 1 min using an electric toothbrush and a standard toothpaste, bleached using a 5.3% hydrogen peroxide solution and cycled for 6 h daily through a tea solution. CIE L* values were obtained after each treatment step using the customized image analysis system and a reflectance spectrophotometer. A statistical analysis was carried out in SPSS. RESULTS: Fleiss' coefficient of reliability for intra-operator repeatability of the image analysis system and spectrophotometry was 0.996 and 0.946 respectively. CIE L* values were consistently higher using the image analysis compared with spectrophotometry, and t-tests for each treatment step showed significant differences (P < 0.05) for the two methods. Limits of agreement between the methods were -27.95 to +2.07, with a 95% confidence of the difference calculated as -14.26 to -11.84. The combined results for all treatment steps showed a significant difference between the methods for the CIE L* values (P < 0.05). CONCLUSION: The image analysis system has proven to be a reliable method for assessment of changes in stain level on extracted teeth. The method has been validated against reflectance spectrophotometry. This method may be used for pilot in vitro studies/trials of oral hygiene and whitening products, before expensive in vivo tests are carried out.
D L Lath; R N Smith; Y H Guan; M Karmo; A H Brook
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Publication Detail:
Type:  Comparative Study; Journal Article; Validation Studies    
Journal Detail:
Title:  International journal of dental hygiene     Volume:  5     ISSN:  1601-5037     ISO Abbreviation:  Int J Dent Hyg     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-07-06     Completed Date:  2008-02-19     Revised Date:  2010-04-29    
Medline Journal Info:
Nlm Unique ID:  101168070     Medline TA:  Int J Dent Hyg     Country:  England    
Other Details:
Languages:  eng     Pagination:  174-9     Citation Subset:  D; IM    
Department of Oral Health and Development, School of Clinical Dentistry, University of Sheffield, Claremont Crescent, UK.
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MeSH Terms
Image Processing, Computer-Assisted*
Photography, Dental*
Reproducibility of Results
Sensitivity and Specificity
Tooth Bleaching
Tooth Discoloration / pathology*
Reg. No./Substance:

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