Document Detail


Measurement of sVEGF R1 and PlGF in serum: comparing prototype assays from Beckman Coulter, Inc. to R&D Systems microplate assays.
MedLine Citation:
PMID:  20482334     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To compare the performance of prototype Access® sVEGF R1 and PlGF automated immunoassays from Beckman Coulter to the Quantikine® microplate ELISA assays by R&D Systems.
METHODS: Samples obtained from pregnant women, non-pregnant women and men were assayed according to manufacturers' instructions.
RESULTS: Compared to the Quantikine assays, the Access assays demonstrated improved precision, increased sensitivity, broader dynamic ranges, and reduced analysis time. The Access assays were found to be specific for free sVEGF R1 and free PlGF.
CONCLUSION: There was good correlation between the Access and Quantikine assays. Superior performance by Access assays may have important prenatal diagnostic implications.
Authors:
Donald Wothe; Emanuel Gaziano; Shiraz Sunderji; Roberto Romero; Juan Pedro Kusanovic; Linda Rogers; Cheryl Hodges-Savola; Sean Roberts; James Wassenberg
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Intramural     Date:  2010-05-19
Journal Detail:
Title:  Hypertension in pregnancy     Volume:  30     ISSN:  1525-6065     ISO Abbreviation:  Hypertens Pregnancy     Publication Date:  2011  
Date Detail:
Created Date:  2011-01-06     Completed Date:  2011-05-24     Revised Date:  2014-07-23    
Medline Journal Info:
Nlm Unique ID:  9421297     Medline TA:  Hypertens Pregnancy     Country:  England    
Other Details:
Languages:  eng     Pagination:  18-27     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Female
Humans
Immunoassay / methods*
Male
Pre-Eclampsia / blood,  diagnosis
Pregnancy
Pregnancy Proteins / blood*,  diagnostic use*
Sensitivity and Specificity
Vascular Endothelial Growth Factor Receptor-1 / blood*,  diagnostic use*
Chemical
Reg. No./Substance:
0/Pregnancy Proteins; 144589-93-5/placenta growth factor; EC 2.7.10.1/Vascular Endothelial Growth Factor Receptor-1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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