Document Detail

Measurement of renin secretion in single perfused rabbit glomeruli.
MedLine Citation:
PMID:  2186637     Owner:  NLM     Status:  MEDLINE    
A new technique is presented that allows the measurement of the renin secretion rate of single rabbit glomeruli during in vitro perfusion at controlled afferent arteriolar perfusion pressure. Rabbit glomeruli with intact afferent arteriole and Bowman's capsule are obtained by microdissection and cannulated with a pipette system that allows continuous afferent arteriolar pressure measurement. The renin secretion rate of 10 glomeruli, perfused at 40 mmHg, was measured in 15-min intervals with an antibody-trapping microassay. Renin secretion rate was low relative to total renin content (1.2-2.0% of content/perfusion h) and increased three- to fivefold in response to isoproterenol (10(-5) M). The afferent arteriole contracted to norepinephrine (10(-5) M) in each instance. This novel, although difficult, technique allows the study of renin release in vitro at controlled perfusion pressure, without the interfering effects of the macula densa, arterial angiotensin II, and the adrenergic nervous system. It should allow a new perspective on issues such as the pressure-flow dependence of renin release and the interaction of the afferent arteriolar endothelium with the renin-secreting juxtaglomerular cells.
H A Bock; M Hermle; A Fiallo; R W Osgood; T A Fried
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  258     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1990 May 
Date Detail:
Created Date:  1990-06-13     Completed Date:  1990-06-13     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  F1460-5     Citation Subset:  IM    
Department of Research, Kantonsspital Basel, Switzerland.
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MeSH Terms
Arterioles / drug effects
Isoproterenol / pharmacology
Kidney Glomerulus / blood supply,  metabolism*
Norepinephrine / pharmacology
Perfusion / instrumentation,  methods
Renin / secretion*
Grant Support
Reg. No./Substance:
51-41-2/Norepinephrine; 7683-59-2/Isoproterenol; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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