| Measurement of renin secretion in single perfused rabbit glomeruli. | |
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MedLine Citation:
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PMID: 2186637 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new technique is presented that allows the measurement of the renin secretion rate of single rabbit glomeruli during in vitro perfusion at controlled afferent arteriolar perfusion pressure. Rabbit glomeruli with intact afferent arteriole and Bowman's capsule are obtained by microdissection and cannulated with a pipette system that allows continuous afferent arteriolar pressure measurement. The renin secretion rate of 10 glomeruli, perfused at 40 mmHg, was measured in 15-min intervals with an antibody-trapping microassay. Renin secretion rate was low relative to total renin content (1.2-2.0% of content/perfusion h) and increased three- to fivefold in response to isoproterenol (10(-5) M). The afferent arteriole contracted to norepinephrine (10(-5) M) in each instance. This novel, although difficult, technique allows the study of renin release in vitro at controlled perfusion pressure, without the interfering effects of the macula densa, arterial angiotensin II, and the adrenergic nervous system. It should allow a new perspective on issues such as the pressure-flow dependence of renin release and the interaction of the afferent arteriolar endothelium with the renin-secreting juxtaglomerular cells. |
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Authors:
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H A Bock; M Hermle; A Fiallo; R W Osgood; T A Fried |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 258 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1990 May |
Date Detail:
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Created Date: 1990-06-13 Completed Date: 1990-06-13 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: F1460-5 Citation Subset: IM |
Affiliation:
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Department of Research, Kantonsspital Basel, Switzerland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arterioles / drug effects Female Isoproterenol / pharmacology Kidney Glomerulus / blood supply, metabolism* Male Norepinephrine / pharmacology Perfusion / instrumentation, methods Rabbits Renin / secretion* Vasoconstriction |
| Grant Support | |
ID/Acronym/Agency:
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DK-17387-16/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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51-41-2/Norepinephrine; 7683-59-2/Isoproterenol; EC 3.4.23.15/Renin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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