| Measurement of renal tissue oxygen tension: systematic differences between fluorescence optode and microelectrode recordings in anaesthetized rabbits. | |
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MedLine Citation:
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PMID: 18223307 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND/AIMS: The validity of fluorescence optodes for measurement of renal cortical tissue oxygen tension was tested by comparison with Clark electrodes. METHODS: We varied renal blood flow and inspired O2 content in anaesthetized rabbits while simultaneously measuring cortical tissue oxygen tension. RESULTS: Cortical oxygen tension varied with inspired O2 content. Fluorescence optode measurements were more tightly distributed than those from a Clark electrode. Cumulative frequency distributions for fluorescence optodes were shifted to the left of those for Clark electrodes. The slope of the relationship between oxygen tension in arterial blood and cortical tissue was less for the fluorescence optode than the Clark electrode. Cortical tissue oxygen tension measurements by these two methods were correlated (r(2) = 0.32; p < 0.001), with no fixed bias but considerable proportional bias. Thus, the slope of the relationship between the two measurements was less than unity (0.57 [0.50-0.69]). CONCLUSION: Cortical oxygen tension values from fluorescence optodes are less variable but proportionally less than those from Clark electrodes. Theoretical considerations suggest that true interstitial oxygen tension lies somewhere between values provided by the two techniques. Nevertheless, the lesser variability of the fluorescence optode technique may aid detection of physiologically significant changes in intrarenal oxygenation. |
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Authors:
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Chai-Ling Leong; Paul M O'Connor; Gabriela A Eppel; Warwick P Anderson; Roger G Evans |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Validation Studies Date: 2008-01-28 |
Journal Detail:
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Title: Nephron. Physiology Volume: 108 ISSN: 1660-2137 ISO Abbreviation: Nephron Physiol Publication Date: 2008 |
Date Detail:
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Created Date: 2008-03-05 Completed Date: 2008-03-20 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 101159772 Medline TA: Nephron Physiol Country: Switzerland |
Other Details:
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Languages: eng Pagination: p11-7 Citation Subset: IM |
Copyright Information:
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(c) 2008 S. Karger AG, Basel |
Affiliation:
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Department of Physiology, Monash University, Melbourne, Vic., Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcholine
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pharmacology Angiotensin II / metabolism Animals Fiber Optic Technology / instrumentation* Fluorescence Kidney Cortex / blood supply, drug effects, metabolism* Male Microelectrodes Oximetry / instrumentation* Oxygen / blood, metabolism* Partial Pressure Rabbits Renal Circulation* / drug effects Reproducibility of Results Time Factors Vasoconstrictor Agents / metabolism Vasodilator Agents / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Vasoconstrictor Agents; 0/Vasodilator Agents; 11128-99-7/Angiotensin II; 51-84-3/Acetylcholine; 7782-44-7/Oxygen |
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