Document Detail


Measurement of prothrombin time and activated partial thromboplastin time in citrated whole blood samples from clinically ill dogs following storage.
MedLine Citation:
PMID:  22860986     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
OBJECTIVES: To assess the reliability of prothrombin time and activated partial thromboplastin time results generated from citrated whole blood samples following short-term storage at room temperature. METHODS: Clotting times were measured in blood samples from 40 dogs that showed a variety of clinical signs. Before measurement of prothrombin time and activated partial thromboplastin time in citrated plasma, whole blood samples were split in three aliquots; one was processed within 30 minutes of collection (fresh) while the remaining two were stored unseparated at room temperature for 24 (24RT) or 48 (48RT) hours. RESULTS: The median prothrombin time for the 24RT (7 seconds) and 48RT (7·2 seconds) samples were not significantly different to those obtained from the fresh (7·1 seconds) samples but the median activated partial thromboplastin time for the 24RT (12·6 seconds) and 48RT (12 seconds) samples were significantly shorter than those obtained from the fresh samples (14·2 seconds). CLINICAL SIGNIFICANCE: Storage of citrated whole blood at room temperature for 24 or 48 hours did not significantly alter the measurement of prothrombin time but resulted in significantly shorter activated partial thromboplastin time results. Extrapolating from these findings, it is proposed that unseparated clinical samples that are submitted to an external diagnostic laboratory for the performance of clotting times, may generate reliable prothrombin time but unreliable activated partial thromboplastin time results.
Authors:
C L Maunder; M Costa; S M Cue; E M Crawford; K Papasouliotis; K F Murphy
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-6
Journal Detail:
Title:  The Journal of small animal practice     Volume:  -     ISSN:  1748-5827     ISO Abbreviation:  J Small Anim Pract     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-6     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0165053     Medline TA:  J Small Anim Pract     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 British Small Animal Veterinary Association.
Affiliation:
School of Veterinary Sciences, University of Bristol, Langford Veterinary Services, Langford House, Langford, Bristol, BS40 5DU; Small Animal Hospital, Langford Veterinary Services, Langford House, Langford, Bristol, BS40 5DU.
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