Document Detail


Measurement of plasma and tissue relaxin concentrations in the pregnant hamster and fetus using a homologous radioimmunoassay.
MedLine Citation:
PMID:  12135888     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A homologous hamster relaxin RIA was developed to evaluate plasma and tissue concentrations of relaxin in the latter half of pregnancy in this species. Relaxin protein and mRNA were localized using antibodies developed to synthetic hamster relaxin and gene-specific molecular probes, respectively. Molecular weight and isoelectric point of the synthetic and native hormones were identical by electrophoretic methods, and synthetic hamster relaxin was active in the mouse interpubic ligament bioassay. Synthetic hormone was used as tracer and standard with rabbit antiserum to the synthetic hormone in the RIA. Relaxin was assayed in blood samples recovered from the retro-orbital plexus on Days 6, 8, 10, 12, 14, 15, and 16 of gestation and on Days 1 and 5 postpartum. Relaxin was first detected on Day 8 of gestation (3.7 +/- 0.6 ng/ml), increased to reach a maximum in the evening of Day 15 (826.0 +/- 124.0 ng/ml), and decreased by Day 16 (day of parturition). Relaxin concentrations were assayed in aqueous extracts of implantation sites (Days 6, 8, and 10) and chorioallantoic placentae (Days 12, 14, and 15). Concentrations were low on Day 6 (0.02 +/- 0.001 microg/g tissue), increased to Day 15 (6.96 +/- 0.86 microg/g tissue), and subsequently declined by the evening of Day 15. Relaxin protein and mRNA were localized to primary and secondary giant trophoblast cells in the chorioallantoic placental trophospongium. However, relaxin protein was not localized in ovaries of pregnant animals or oviductal tissues of cycling animals. Significant quantities of relaxin were detected in the serum of fetal hamsters recovered on Day 15.
Authors:
Randall H Renegar; Charles R Owens
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biology of reproduction     Volume:  67     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-07-23     Completed Date:  2003-01-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  500-5     Citation Subset:  IM    
Affiliation:
Department of Anatomy and Cell Biology, Brody School of Medicine, East Carolina University, 600 Moye Boulevard, Greenville, NC 27858, USA. renegarra@mail.ecu.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibody Specificity
Chorion / chemistry
Cricetinae
DNA, Complementary / chemistry
Female
Fetus / metabolism*
Immunohistochemistry
In Situ Hybridization
Mesocricetus
Molecular Weight
Placenta / chemistry
Pregnancy
RNA, Messenger / biosynthesis,  genetics
Radioimmunoassay
Recombinant Proteins / chemistry
Relaxin / analysis*,  blood,  chemistry
Tissue Distribution
Trophoblasts / cytology,  metabolism
Grant Support
ID/Acronym/Agency:
HD 23481/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/RNA, Messenger; 0/Recombinant Proteins; 9002-69-1/Relaxin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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