| Measurement of plasma protein synthesis rate in infant pig: an investigation of alternative tracer approaches. | |
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MedLine Citation:
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PMID: 8048626 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To devise a new method to measure plasma protein synthesis, we tested the hypothesis that, when [U-13C]glucose is used to produce [U-13C]alanine, plasma pyruvate and alanine will be in isotopic equilibrium with the alanine used to synthesize plasma proteins. The incorporation of labeled leucine, lysine, and alanine into very-low-density lipoprotein (VLDL) apolipoprotein B (apoB)-100, albumin, and fibrinogen was measured in seven infant pigs by infusing [U-13C]glucose, [2H3]leucine, and [2H4]lysine. The plateau enrichments of plasma alanine (2.29 +/- 0.29), pyruvate (2.5 +/- 0.33), and apoB-alanine (2.33 +/- 0.25) were not different. The fractional synthesis rates of fibrinogen and albumin calculated using the isotopic enrichments of apoB-bound lysine, leucine, and alanine as the precursor were similar to those based on plasma alanine. These results suggest that the intrahepatic precursor alanine pool and plasma alanine were in isotopic equilibrium. Thus plasma protein synthesis can be measured by infusing [U-13C]glucose and using plasma alanine as precursor. |
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Authors:
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F Jahoor; D G Burrin; P J Reeds; M Frazer |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 267 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1994 Jul |
Date Detail:
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Created Date: 1994-09-01 Completed Date: 1994-09-01 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: R221-7 Citation Subset: IM |
Affiliation:
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Department of Pediatrics, Baylor College of Medicine, Houston, Texas 77030. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alanine
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metabolism Animals Animals, Newborn / metabolism* Apolipoprotein B-100 Apolipoproteins B / metabolism Blood Proteins / biosynthesis* Carbon Isotopes Deuterium Female Fibrinogen / metabolism Leucine / metabolism Liver / metabolism Lysine / metabolism Male Serum Albumin / metabolism Swine |
| Grant Support | |
ID/Acronym/Agency:
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DK-41764/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Apolipoprotein B-100; 0/Apolipoproteins B; 0/Blood Proteins; 0/Carbon Isotopes; 0/Serum Albumin; 56-41-7/Alanine; 56-87-1/Lysine; 61-90-5/Leucine; 7782-39-0/Deuterium; 9001-32-5/Fibrinogen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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