Document Detail


Measurement of phosphorothioate oligodeoxynucleotide antisense transport across the blood-brain barrier.
MedLine Citation:
PMID:  21922419     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Phosphorothioate oligodeoxynucleotides (PODNs) can act as antisense molecules, knocking down proteins. Many PODNs have the unusual characteristic of being transported across the blood-brain barrier by a saturable system. This means that PODNs injected intravenously can accumulate in the central nervous system in quantities sufficient to knock down proteins in brain and the blood-brain barrier. A critical step in the development of PODNs that can be administered peripherally and knockdown proteins in the central nervous system is to determine the relation to the blood-brain barrier, specifically, does the PODN cross the blood-brain barrier and, if so, how fast and to what degree.
Authors:
William A Banks
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  789     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2011  
Date Detail:
Created Date:  2011-09-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  337-42     Citation Subset:  IM    
Affiliation:
Geriatrics Research, Education, and Clinical Center, Puget Sound Health Care System, Seattle, WA, USA, wabanks1@u.washington.edu.
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