| Measurement of penicillamine and N-acetylcysteine in human blood by high-performance liquid chromatography and electrochemical detection. | |
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MedLine Citation:
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PMID: 3958085 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A rapid and precise high-performance liquid chromatographic assay for both N-acetylcysteine and penicillamine in blood samples is described using selective reductive electrochemical detection and a high-efficiency C18 reversed-phase column. The use of an internal standard compensated for changes in detector responses during a run and for variable sample recovery. The detection limits for N-acetylcysteine and penicillamine were 25 and 10 ng/ml, respectively, using 500-microliters blood samples. Reproducibility of measurement for both thiols was excellent. This method allows routine monitoring of blood levels and pharmacokinetic studies with N-acetylcysteine and penicillamine. |
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Authors:
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O H Drummer; N Christophidis; J D Horowitz; W J Louis |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of chromatography Volume: 374 ISSN: 0021-9673 ISO Abbreviation: J. Chromatogr. Publication Date: 1986 Jan |
Date Detail:
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Created Date: 1986-04-28 Completed Date: 1986-04-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0427043 Medline TA: J Chromatogr Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 251-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcysteine
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blood* Arthritis, Rheumatoid / blood Chromatography, High Pressure Liquid Drug Stability Electrochemistry Humans Kinetics Penicillamine / blood* Sulfhydryl Compounds / blood |
| Chemical | |
Reg. No./Substance:
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0/Sulfhydryl Compounds; 52-67-5/Penicillamine; 616-91-1/Acetylcysteine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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