Document Detail


Measurement of penicillamine and N-acetylcysteine in human blood by high-performance liquid chromatography and electrochemical detection.
MedLine Citation:
PMID:  3958085     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A rapid and precise high-performance liquid chromatographic assay for both N-acetylcysteine and penicillamine in blood samples is described using selective reductive electrochemical detection and a high-efficiency C18 reversed-phase column. The use of an internal standard compensated for changes in detector responses during a run and for variable sample recovery. The detection limits for N-acetylcysteine and penicillamine were 25 and 10 ng/ml, respectively, using 500-microliters blood samples. Reproducibility of measurement for both thiols was excellent. This method allows routine monitoring of blood levels and pharmacokinetic studies with N-acetylcysteine and penicillamine.
Authors:
O H Drummer; N Christophidis; J D Horowitz; W J Louis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography     Volume:  374     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1986 Jan 
Date Detail:
Created Date:  1986-04-28     Completed Date:  1986-04-28     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  251-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acetylcysteine / blood*
Arthritis, Rheumatoid / blood
Chromatography, High Pressure Liquid
Drug Stability
Electrochemistry
Humans
Kinetics
Penicillamine / blood*
Sulfhydryl Compounds / blood
Chemical
Reg. No./Substance:
0/Sulfhydryl Compounds; GNN1DV99GX/Penicillamine; WYQ7N0BPYC/Acetylcysteine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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