| Measurement of paralytic shellfish toxins in molluscan extracts: comparison of the microtitre plate saxiphilin and sodium channel radioreceptor assays with mouse bioassay, HPLC analysis and a commercially available cell culture assay. | |
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MedLine Citation:
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PMID: 11665738 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study compared five methods of measuring paralytic shellfish toxins (PSTs) including the long-used mouse lethality bioassay, a commercially available cell culture test (MIST Quantification kit), HPLC analysis, and two newly developed radioreceptor assays utilizing mammalian sodium channels and saxiphilin. Methods were challenged with toxic shellfish extracts prepared according to the AOAC official method. The best correlations between predicted toxicity values being 0.9 or better, were those between HPLC analysis when compared with both radioreceptor assays and the mouse lethality bioassay, as well as that between the saxiphilin and the sodium channel radioreceptor assays. In all cases, statistically significant correlations existed between the toxicity measurements of the same extracts. The ratios between some methods were not unitary as measured by the slopes of the regression lines used for correlation analyses. HPLC analysis predicted more toxicity than all of the bioassays. The saxiphilin assay underestimated toxicity relative to the mouse bioassay, the MIST kit determinations and the sodium channel assay. The sodium channel assay predicted there to be less toxicity than the mouse bioassay and the MIST kit. Of all of the techniques used, the MIST kit correlation with the mouse bioassay was nearest to one. Each method possesses differentt virtues and it may be that a multi-method approach would harness the benefits of each method for various aspects of a shellfish testing regime. |
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Authors:
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L E Llewellyn; J Doyle; J Jellett; R Barrett; C Alison; C Bentz; M Quilliam |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Food additives and contaminants Volume: 18 ISSN: 0265-203X ISO Abbreviation: Food Addit Contam Publication Date: 2001 Nov |
Date Detail:
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Created Date: 2001-10-22 Completed Date: 2001-12-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8500474 Medline TA: Food Addit Contam Country: England |
Other Details:
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Languages: eng Pagination: 970-80 Citation Subset: IM |
Affiliation:
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Australian Institute of Marine Science, PMB 3, Townsville MC, Qld. L.Llewellyn@aims.gov.au |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amphibian Proteins Animals Biological Assay / methods, standards Carrier Proteins Cell Culture Techniques / methods, standards Chromatography, High Pressure Liquid / methods Marine Toxins / analysis* Mice Mollusca* Radioligand Assay / methods, standards Reproducibility of Results Sensitivity and Specificity Shellfish* Sodium Channels Titrimetry / methods, standards |
| Chemical | |
Reg. No./Substance:
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0/Amphibian Proteins; 0/Carrier Proteins; 0/Marine Toxins; 0/Sodium Channels; 0/saxitoxin-binding protein, Rana catesbeiana |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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