Document Detail

Measurement of ovine pregnancy-associated glycoprotein (PAG) during early pregnancy in Lacaune sheep.
MedLine Citation:
PMID:  17506803     Owner:  NLM     Status:  MEDLINE    
This study describes ovine pregnancy-associated glycoprotein (ovPAG) concentrations in 20 Lacaune sheep during early pregnancy. Measurements were performed by using semi-purified ovPAG as standard, tracer and immunogens for antibody production in rabbits. Antisera R780 (against ovPAG(57+59kDa)) and R805 (against ovPAG5(58+61kDa)) were used respectively in RIA-780 and RIA-805. Blood samples were collected at days 0, 18, 20, 22 and 25 after artificial insemination. From day 18 after breeding onward, the mean ovPAG concentration was significantly higher (p < 0.001) in plasma samples from pregnant ewes (n = 17) than in non-pregnant ones (n = 3). The specific activity of the tracer was 11 760 Ci/mmol in RIA-780 and 14 900 Ci/mmol in RIA-805. The minimal detection limits for RIA-780 and RIA-805 were 0.2 ng/ml and 0.3 ng/ml, respectively. The intra-assay CV of samples with low (1.0 ng/ml), medium (2.5 ng/ml) and high (4.0 ng/ml) PAG concentrations were 3%, 6% and 9% for RIA-780 and 8%, 9% and 5% for RIA-805. The inter-assay CV in the same samples were 13%, 12% and 7% for RIA-780 and 13%, 11% and 5% for RIA-805. The recovery was higher than 95% in both assays. No cross-reaction was observed with members of aspartic proteinase family as well as with other tested proteins. In both RIA-780 and RIA-805, inhibition of the binding of the tracer by antisera was parallel between standard curve and serial dilutions of pregnant ewe samples. In conclusion, the two homologous RIA systems are suitable for early quantification of ovPAG concentrations in ewe plasma samples from day 18 after breeding.
B El Amiri; A Karen; J Sulon; N Melo de Sousa; A V Alvarez-Oxiley; Y Cognié; O Szenci; J F Beckers
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproduction in domestic animals = Zuchthygiene     Volume:  42     ISSN:  0936-6768     ISO Abbreviation:  Reprod. Domest. Anim.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-05-17     Completed Date:  2007-06-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9015668     Medline TA:  Reprod Domest Anim     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  257-62     Citation Subset:  IM    
Faculty of Veterinary Medicine, University of Liege, Liege, Belgium.
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MeSH Terms
Gestational Age
Predictive Value of Tests
Pregnancy Proteins / blood*
Pregnancy Tests / methods,  veterinary*
Pregnancy, Animal / blood*
Radioimmunoassay / veterinary
Sensitivity and Specificity
Sheep / blood*
Reg. No./Substance:
0/Pregnancy Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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