Document Detail

Measurement of nitric oxide in hippocampal slices: induction with nitroso compounds and the effect of depolarization.
MedLine Citation:
PMID:  15053255     Owner:  NLM     Status:  MEDLINE    
Nitric oxide not only acts as a messenger for different physiological processes, but also mediates neurotoxicity associated with a variety of neurological disorders including epilepsy. The molecular mechanisms behind these actions are unclear. In this study, we aimed to detect relative amounts of NO released from rat hippocampal slices by chemiluminescence measurements under NMDA stimulation and spontaneous depolarization conditions. Hippocampal slices were preferred because of their functional integrity useful in simulating in vivo conditions. The reliability of the system was verified by administering increasing concentrations of a NO donor sodium nitroprusside in different redox milieu and a NO scavenger, carboxy-2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide (carboxy PTIO). The redox versatility of NO allows interconversion from neuroprotective to neurotoxic species by a change in the ambient redox milieu. We have quantitated NO formed under NMDA stimulation and spontaneous depolarization conditions, and showed that depolarization increased NO formation and was excitotoxic for the neural tissue.
Goncagül Haklar; Hale Saybaşili; Meral Yüksel; A Süha Yalçin
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Acta neurobiologiae experimentalis     Volume:  63     ISSN:  0065-1400     ISO Abbreviation:  Acta Neurobiol Exp (Wars)     Publication Date:  2003  
Date Detail:
Created Date:  2004-03-31     Completed Date:  2004-04-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1246675     Medline TA:  Acta Neurobiol Exp (Wars)     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  319-25     Citation Subset:  IM    
Department of Biochemistry, School of Medicine, Marmara University, Istanbul, Turkey.
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MeSH Terms
Acridines / metabolism
Benzoates / pharmacology
Chemiluminescent Measurements
Free Radical Scavengers / pharmacology
Hippocampus / chemistry,  drug effects,  metabolism*
Imidazoles / pharmacology
L-Lactate Dehydrogenase / metabolism
N-Methylaspartate / pharmacology
Nitric Oxide / analysis,  metabolism*
Nitric Oxide Donors / pharmacology
Nitroprusside / pharmacology
Nitroso Compounds / pharmacology*
Rats, Sprague-Dawley
Reg. No./Substance:
0/Acridines; 0/Benzoates; 0/Free Radical Scavengers; 0/Imidazoles; 0/Nitric Oxide Donors; 0/Nitroso Compounds; 10102-43-9/Nitric Oxide; 145757-47-7/1,3-dihydroxy-4,4,5,5-tetramethyl-2-(4-carboxyphenyl)tetrahydroimidazole; 15078-28-1/Nitroprusside; 2315-97-1/10,10'-dimethyl-9,9'-biacridinium; 6384-92-5/N-Methylaspartate; EC Dehydrogenase

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