Document Detail


Measurement of neonatal equine immunoglobulins for assessment of colostral immunoglobulin transfer: comparison of single radial immunodiffusion with the zinc sulfate turbidity test, serum electrophoresis, refractometry for total serum protein, and the sodium sulfite precipitation test.
MedLine Citation:
PMID:  413817     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Four procedures for assessment of adequacy of colostral immunoglobulin (Ig) transfer in foals were evaluated. Results of zinc sulfate turbidity test, serum electrophoresis, total serum protein refractometry, and sodium sulfite precipitation test were compared with immunoglobulin G content determined by single radial immunodiffusion. The zinc sulfate turbidity test gave acceptable results for IgG, except that hemolyzed serum samples gave higher than expected values. A correction factor for hemolyzed serum was found to be useful. Serum electrophoresis was a satisfactory method of estimating IgG content. Total serum protein values may not be a valid basis for estimating IgG content, inasmuch as postsuckling total protein values were found to decrease in some foals in which passive transfer of IgG had been adequate. Sodium sulfite precipitation reactions were too unpredictable to be of value for determination of neonatal IgG concentration.
Authors:
G E Rumbaugh; A A Ardans; D Ginno; A Trommershausen-Smith
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of the American Veterinary Medical Association     Volume:  172     ISSN:  0003-1488     ISO Abbreviation:  J. Am. Vet. Med. Assoc.     Publication Date:  1978 Feb 
Date Detail:
Created Date:  1978-03-21     Completed Date:  1978-03-21     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7503067     Medline TA:  J Am Vet Med Assoc     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  321-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Blood Protein Electrophoresis / methods
Chemical Precipitation
Colostrum / immunology*
Female
Hemolysis
Horses / immunology*
Immunization, Passive*
Immunodiffusion
Immunoglobulin G / analysis
Immunoglobulins / analysis*
Nephelometry and Turbidimetry / methods
Sulfates / diagnostic use
Zinc / diagnostic use
Chemical
Reg. No./Substance:
0/Immunoglobulin G; 0/Immunoglobulins; 0/Sulfates; 7440-66-6/Zinc

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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