| Measurement of the mobility of all-trans-retinol with two-photon fluorescence recovery after photobleaching. | |
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MedLine Citation:
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PMID: 20552425 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mobility of all-trans-retinol makes a crucial contribution to the rate of the reactions in which it participates. This is even more so because of its low aqueous solubility, which makes the presence of carrier proteins and the spatial arrangement of cellular membranes especially relevant. In rod photoreceptor outer segments, all-trans-retinol is generated after light exposure from the reduction of all-trans-retinal that is released from bleached rhodopsin. The mobility of all-trans-retinol in rod outer segments was measured with fluorescence recovery after photobleaching (FRAP), using two-photon excitation of its fluorescence. The values of the lateral and axial diffusion coefficients indicate that most of the all-trans-retinol in rod outer segments move unrestricted and without being aided by carriers. |
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Authors:
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Yiannis Koutalos |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 652 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-06-16 Completed Date: 2010-09-09 Revised Date: 2011-07-20 |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 115-27 Citation Subset: IM |
Affiliation:
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Departments of Ophthalmology and Neurosciences, Medical University of South Carolina, Charleston, SC 29425, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anura Cell Separation Diffusion Fluorescence Recovery After Photobleaching / methods* Movement* Photons* Rod Cell Outer Segment / metabolism Vitamin A / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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R01 EY014850-07/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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11103-57-4/Vitamin A |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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