Document Detail

Measurement of mitochondrial respiration in trophoblast culture.
MedLine Citation:
PMID:  22336334     Owner:  NLM     Status:  MEDLINE    
Pregnancy is a state of oxidative stress, which becomes exaggerated under pathological conditions, such as preeclampsia, IUGR, diabetes and obesity, where placental mitochondrial dysfunction is observed. The majority of investigations utilize isolated mitochondria when measuring mitochondrial activity in placenta. However, this does not provide a complete physiological readout of mitochondrial function. This technical note describes a method to measure respiratory function in intact primary syncytiotrophoblast from human term placenta.
A Maloyan; J Mele; B Muralimanohara; L Myatt
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Publication Detail:
Type:  Journal Article     Date:  2012-02-14
Journal Detail:
Title:  Placenta     Volume:  33     ISSN:  1532-3102     ISO Abbreviation:  Placenta     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-04-02     Completed Date:  2012-08-03     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  8006349     Medline TA:  Placenta     Country:  England    
Other Details:
Languages:  eng     Pagination:  456-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2012. Published by Elsevier Ltd.
Center for Pregnancy and Newborn Research, Department of Obstetrics and Gynecology, University of Texas Health Science Center San Antonio, Mail Code 7836, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA.
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MeSH Terms
Anoxia / metabolism
Cell Culture Techniques*
Cell Respiration
Cells, Cultured
Mitochondria / metabolism*
Oxygen Consumption*
Trophoblasts / metabolism*
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