Document Detail


Measurement of methylglyoxal in rat tissues by electrospray ionization mass spectrometry and liquid chromatography.
MedLine Citation:
PMID:  15767209     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
INTRODUCTION: Increased methylglyoxal formation due to insulin resistance has been implicated in the development of essential hypertension and in type-2 diabetic complications in animal models. Methylglyoxal is a highly reactive aldehyde, which binds sulfhydryl and amino groups of membrane proteins forming conjugates, advanced glycation end products (AGEs), which alter membrane function, leading to increased blood pressure and oxidative stress. We have shown elevated aldehyde conjugates in tissues of hypertensive rats which may be formed primarily from methylglyoxal. Our objective was to develop a specific method to measure methylglyoxal in rat tissues. METHOD: This method involves preparation of plasma, blood and tissue homogenates, solid phase extraction of methylglyoxal, derivitization using o-phenylenediamine, further purification of derivatized products by solid phase extraction and quantification by electrospray ionization liquid chromatography mass spectrometry (ESI/LC/MS). RESULTS: Methylglyoxal was highest in aorta followed by heart, liver, kidney and blood in that order in Sprague-Dawley rats. Levels of methylglyoxal in plasma were about an order of magnitude lower than that in tissues, but above the concentration used for the lowest calibration standard. DISCUSSION: We have successfully developed an ESI/LC/MS method for quantification of methylglyoxal in rat tissues. The high selectivity of this method offers an advantage over other methods based on fluorescence. This method will allow the evaluation of methylglyoxal in essential hypertension and type-2 diabetes.
Authors:
E W Randell; S Vasdev; V Gill
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of pharmacological and toxicological methods     Volume:  51     ISSN:  1056-8719     ISO Abbreviation:  J Pharmacol Toxicol Methods     Publication Date:    2005 Mar-Apr
Date Detail:
Created Date:  2005-03-15     Completed Date:  2005-07-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9206091     Medline TA:  J Pharmacol Toxicol Methods     Country:  United States    
Other Details:
Languages:  eng     Pagination:  153-7     Citation Subset:  IM    
Affiliation:
Department of Laboratory Medicine, Faculty of Medicine, Memorial University of Newfoundland, St. John's, Newfoundland, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aorta / chemistry
Chromatography, Liquid*
Kidney / chemistry
Liver / chemistry
Male
Mass Spectrometry*
Molecular Structure
Myocardium / chemistry
Pyruvaldehyde / analysis*,  blood*,  chemistry
Rats
Rats, Sprague-Dawley
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization*
Chemical
Reg. No./Substance:
78-98-8/Pyruvaldehyde

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