Document Detail

Measurement of melatonin and 6-sulphatoxymelatonin.
MedLine Citation:
PMID:  23996364     Owner:  NLM     Status:  In-Data-Review    
Melatonin is an indole hormone secreted by the pineal gland during the hours of darkness in a normally entrained individual. There is a clear circadian rhythm in its production with low levels during the day and a peak in the early hours of the morning. The timing of sample collection is crucial and single time point measurements are of little use. Measurement of melatonin or its major metabolite, 6-sulphatoxymelatonin, is normally carried out to determine the timing of an individual's internal body clock and whether it is synchronized to the 24 h day. Misalignment of the clock or disruption of the rhythm can lead to difficulties in sleeping and health problems such as are associated with jet-lag or shift work. Both melatonin and 6-sulphatoxymelatonin can be measured by RIA or ELISA. Details of sample collection and preparation and the assay procedures are described.
Benita Middleton
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  1065     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2013-09-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  171-99     Citation Subset:  IM    
Chronobiology Group, Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey, UK.
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