| Measurement of intraventricular pressure and cardiac performance in the intact closed-chest anesthetized mouse. | |
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MedLine Citation:
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PMID: 9087586 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To fully utilize the potential of newly developed mouse models with specific genetic mutations, it is necessary to study the functional consequences of genetic manipulation in the fully intact animal. To this end, the purpose of the present study was to develop and validate a methodology for the study of myocardial performance in the fully intact, closed-chest mouse. Left ventricular function was evaluated in euthyroid, hypothyroid, and hyperthyroid mice, animals with well-documented alterations in myocardial function. The mice were anesthetized and instrumented with polyethylene catheters in the right femoral artery and vein and with a Millar MIKRO-TIP transducer in the left ventricle via the right carotid artery. Structural and functional evidence suggested that the instrumentation procedure did not cause myocardial damage, valvular insufficiency, or aortic obstruction. Isovolumic indexes of myocardial contractility derived from the left ventricular pressure pulse and its first derivative demonstrated a 40% increase in contractility in the hyperthyroid animals and a 40% decrease in contractility in the hypothyroid animals. Similar differences in the indexes of relaxation were observed. Furthermore, isoproterenol dose-response relationships of these contractile parameters were blunted in the hypothyroid animals and augmented in the hyperthyroid animals compared with euthyroid control animals. Given the small size of the mouse and the high frequency of the cardiac cycle, these data demonstrate the feasibilty of combining a high-fidelity, microtip manometer with a high-speed data-acquisition system to obtain faithful recordings of cardiac performance in the fully intact mouse. |
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Authors:
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J N Lorenz; J Robbins |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The American journal of physiology Volume: 272 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1997 Mar |
Date Detail:
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Created Date: 1997-04-28 Completed Date: 1997-04-28 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: H1137-46 Citation Subset: IM |
Affiliation:
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Department of Molecular and Cellular Physiology, University of Cincinnati, Ohio 45267-0576, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anesthesia, General Animals Blood Pressure / drug effects Dose-Response Relationship, Drug Female Heart / drug effects, physiology*, physiopathology Heart Rate / drug effects Heart Ventricles Hemodynamics* / drug effects Hyperthyroidism / physiopathology* Hypothyroidism / physiopathology* Isoproterenol / pharmacology Male Mice Mice, Inbred Strains Propranolol / pharmacology Reference Values Reproducibility of Results Ventricular Function, Left / drug effects |
| Chemical | |
Reg. No./Substance:
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525-66-6/Propranolol; 7683-59-2/Isoproterenol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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