Document Detail


Measurement of interferon-tau (IFN-tau) stimulated gene expression in blood leukocytes for pregnancy diagnosis within 18-20d after insemination in dairy cattle.
MedLine Citation:
PMID:  20554404     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective was to diagnose pregnancy within 18d after insemination by measuring interferon-tau (IFN-tau)-stimulated genes (ISG) expression in circulatory leukocytes. Based on microarray results, three genes were selected [2'-5' oligoadenylate synthetase 1(Oas1), myxovirus resistance gene 2 (Mx2), and interferon-stimulated gene 15kDa protein (Isg15)] because they were known to be interferon-stimulated genes (ISG) and were also differentially expressed in the samples. The respective genes were assayed by using real time reverse transcriptase polymerase chain reaction (RTPCR). In the first experiment, RNA was isolated from leukocytes of pregnant (n=5) and non-pregnant (n=15) dairy cows on each of d 14, 16, 18, and 20 after insemination. The ISG expression (Mx2 and Isg15) was greater in pregnant compared with non-pregnant cows on d 18 and 20. In the second experiment, we attempted to define the shortest interval to pregnancy detection by measuring ISG on d 17 (Exp. 2A) or d 18 (Exp. 2B). For Exp. 2A, blood was collected from pregnant (n=16) and non-pregnant (n=16) cows on d 17 after insemination. On d 17, pregnant cows had greater Mx2 and Oas1 but Receiver Operator Characteristic (ROC) curves showed that the increase was too small to develop a suitable cut-off for a reliable pregnancy test. For Exp. 2B, blood samples were collected from pregnant (n=21) and non-pregnant (n=21) cows on d 18 after insemination. For both Mx2 and Oas1, primiparous pregnant cows had greater ISG expression than primiparous non-pregnant cows on d 18. For multiparous cows, however, pregnant and non-pregnant cows were similar for ISG expression on d 18. The possibility of correcting for pre-insemination ISG expression was examined in a final series of experiments. Blood samples were collected from cows (n=54) and heifers (n=24) during the luteal phase preceding insemination. A second sample was collected from the same cows [pregnant (n=13) and non-pregnant (n=41)] and heifers [pregnant (n=17) and non-pregnant (n=7)] 18d after insemination. The ratio of the second sample (after insemination) to the first sample (before insemination) minimally increased the sensitivity of the ISG test for pregnancy detection. There was a large increase in ISG expression on d 18 of pregnancy in heifers (approximately 1 year of age) that could be measured for a reliable pregnancy test. The conclusion from the studies is that a reliable ISG-based method for pregnancy detection could be applied as early as d 18 but only in heifers. Older cows have a lesser response that reduces the sensitivity of the ISG test on or before d 18.
Authors:
J C Green; C S Okamura; S E Poock; M C Lucy
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Publication Detail:
Type:  Controlled Clinical Trial; Journal Article     Date:  2010-05-20
Journal Detail:
Title:  Animal reproduction science     Volume:  121     ISSN:  1873-2232     ISO Abbreviation:  Anim. Reprod. Sci.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-12     Completed Date:  2010-11-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7807205     Medline TA:  Anim Reprod Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  24-33     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Elsevier B.V. All rights reserved.
Affiliation:
Division of Animal Sciences, University of Missouri, 158 Animal Science Research Center, 920 East Campus Drive, Columbia, MO 65211, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Dairying
Female
Gene Expression / drug effects*
Gene Expression Profiling
Gestational Age
Insemination, Artificial / veterinary
Interferon Type I / pharmacology*
Leukocytes / drug effects*,  metabolism
Oligonucleotide Array Sequence Analysis
Pregnancy
Pregnancy Proteins / pharmacology*
Pregnancy Tests / methods*,  veterinary
Pregnancy, Animal* / blood,  genetics
Chemical
Reg. No./Substance:
0/Interferon Type I; 0/Pregnancy Proteins; 0/trophoblastin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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