| Measurement of hyaluronidase activity in normal human serum. | |
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MedLine Citation:
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PMID: 8807545 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A detailed evaluation of the assay for serum hyaluronidase (HAE) activity originally developed by Bonner and Cantey [W.M. Bonner, Jr. and E.Y. Cantey, Clin. Chim. Acta, 13 (1966) 746-752] is described, together with studies of its precision. The method is based on the liberation of saccharides with N-acetylglucosamine (NAG) end-groups from hyaluronic acid. The NAG is quantitated by heating with alkaline tetraborate to form an intermediate which reacts with p-dimethylaminobenzaldehyde in acidic medium to form a coloured product. The optimised assay, which requires less that 50 microliters of serum, was used to study the HAE activity of 70 normal sera. The mean HAE activity was 17.1 mumol NAG min-1 l-1 (range 11.5-27.0 mumol NAG min-1 l-1); there was no significant difference with age (t = 1.65, 0.5 > P > 0.1) or sex (t = 0.33, P > 0.5). |
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Authors:
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C R Wilkinson; L M Bower; C Warren |
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Publication Detail:
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Type: Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of pharmaceutical and biomedical analysis Volume: 14 ISSN: 0731-7085 ISO Abbreviation: J Pharm Biomed Anal Publication Date: 1996 Apr |
Date Detail:
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Created Date: 1996-12-05 Completed Date: 1996-12-05 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8309336 Medline TA: J Pharm Biomed Anal Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 707-12 Citation Subset: IM |
Affiliation:
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Department of Chemical Pathology, Torbay Hospital, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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9,10-Dimethyl-1,2-benzanthracene Acetylglucosamine / chemistry Adult Aged Aged, 80 and over Aging Borates Colorimetry Evaluation Studies as Topic Female Humans Hyaluronic Acid / chemistry Hyaluronoglucosaminidase / blood* Hydrogen-Ion Concentration Indicators and Reagents Male Middle Aged Sex Characteristics |
| Chemical | |
Reg. No./Substance:
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0/Borates; 0/Indicators and Reagents; 12258-53-6/tetraborate; 57-97-6/9,10-Dimethyl-1,2-benzanthracene; 7512-17-6/Acetylglucosamine; 9004-61-9/Hyaluronic Acid; EC 3.2.1.35/Hyaluronoglucosaminidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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