Document Detail

Measurement of free IGF-I saliva levels: perspectives in the detection of GH/IGF axis in athletes.
MedLine Citation:
PMID:  17321513     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: To examine an immunoassay for measuring free IGF-I in a saliva specimen (free sIGF-I) and to study the levels in relation to the training conditions comparing young athletes and sedentary females. DESIGN AND METHODS: The analysis was carried out by modifying a commercial kit for plasma matrix to measure the free sIGF-I. The plasma free and total IGF-I fractions, hGH and salivary total proteins were also measured. Saliva and blood specimens were collected from 15 well-trained young female volleyball athletes and from a control group of 14 young sedentary females. RESULTS: The calibration curve to assay free sIGF-I covered the range 0.05-5.00 microg/L. The detection limit was 0.07 microg/L. The within-run and between-run imprecision CVs were 10% and 13% respectively. The average recovery was 88%. Free sIGF-I, measured in 15 athletes and in 14 young sedentary females, was 0.10+/-0.03 and 0.20+/-0.05 micarog/L respectively (p<0.001). CONCLUSIONS: There were decreased levels of free sIGF-I in well-trained athletes, compared with sedentary females. This decrease could be related to a greater tissue requirement by the active muscle subjected to intense exercise for several days.
G Antonelli; E Cappellin; R Gatti; S Chiappin; P Spinella; E F De Palo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-01-26
Journal Detail:
Title:  Clinical biochemistry     Volume:  40     ISSN:  0009-9120     ISO Abbreviation:  Clin. Biochem.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-04-23     Completed Date:  2007-07-10     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0133660     Medline TA:  Clin Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  545-50     Citation Subset:  IM    
Section of Clinical Biochemistry, Department of Medical Diagnostic Sciences and Spec. Ther., University of Padua, c/o ex Istituto di Semeiotica Medica Via Ospedale 105, 35128 Padova, Italy.
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MeSH Terms
Case-Control Studies
Exercise / physiology*
Human Growth Hormone / analysis,  blood
Immunoassay / methods
Insulin-Like Growth Factor I / analysis*
Reproducibility of Results
Saliva / chemistry*
Salivary Proteins and Peptides / analysis*
Reg. No./Substance:
0/Salivary Proteins and Peptides; 12629-01-5/Human Growth Hormone; 67763-96-6/Insulin-Like Growth Factor I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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