| Measurement of fluid secretion from intact airway submucosal glands. | |
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MedLine Citation:
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PMID: 21547728 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Human airways are kept sterile by a mucosal innate defense system that includes mucus secretion. Mucus is secreted in healthy upper airways primarily by submucosal glands and consists of defense molecules mixed with mucins, electrolytes, and water and is also a major component of sputum. Mucus traps pathogens and mechanically removes them via mucociliary clearance while inhibiting their growth via molecular (e.g., lysozyme) and cellular (e.g., neutrophils, macrophages) defenses. Fluid secretion rates of single glands in response to various mediators can be measured by trapping the primary gland mucus secretions in an oil layer, where they form spherical bubbles that can be optically measured at any desired interval to provide detailed temporal analysis of secretion rates. The composition and properties of the mucus (e.g., solids, viscosity, pH) can also be determined. These methods have now been applied to mice, ferrets, cats, pigs, sheep, and humans, with a main goal of comparing gland secretion in control and CFTR-deficient humans and animals. |
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Authors:
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Jeffrey J Wine; Nam Soo Joo; Jae Young Choi; Hyung-Ju Cho; Mauri E Krouse; Jin V Wu; Monal Khansaheb; Toshiya Irokawa; Juan Ianowski; John W Hanrahan; Alan W Cuthbert; Kim V Tran |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 742 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2011 |
Date Detail:
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Created Date: 2011-05-06 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 93-112 Citation Subset: IM |
Affiliation:
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Cystic Fibrosis Research Laboratory, Stanford University, 94305-2130, Stanford, CA, USA, wine@stanford.edu. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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