Document Detail

Measurement of factor VII and of activated factor VII in healthy individuals and in prothrombin complex concentrates.
MedLine Citation:
PMID:  9219329     Owner:  NLM     Status:  MEDLINE    
We established reference ranges for factor VII clotting activity (FVII:C), factor VII amidolytic activity (FVII:AM), and activated factor VII (FVIIa) in 102 healthy individuals. The reference ranges were 65-160 U/100 ml, 70-165 U/100 ml, and 30-170 mU/ml, respectively (2.5 and 97.5 percentiles). Freezing and thawing of the plasma samples had no influence on the assay results. Due to the small sample size, the results were not influenced by gender, age, smoking habits, and oral contraceptive use. The plasma levels of FVII:C, FVII:AM, and FVIIa were significantly correlated with each other. The significant correlation between FVIIa and FVII:AM indicates that FVIIa is not completely independent of circulating FVII mass. There was also a significant, though weak, correlation between FVIIa and FVII:C/FVII:AM ratios. Sixteen batches of prothrombin complex concentrates (PCC) from 3 manufacturers were also analysed. FVIIa could be detected in all preparations, with considerable variations from batch to batch. In contrast to the results obtained in plasma from normal individuals, there was a close correlation between FVIIa and FVII:C/FVII:AM ratios. The preparations could be characterized by their FVII and FVIIa potencies and by their FVII:C/FVII:AM ratios. In PCC, FVII:C was very strongly correlated with FVIIa, whereas no significant correlation was observed between FVII:AM and FVII:C and between FVII:AM and FVIIa, respectively. These results demonstrate that the FVII:C assay used is sensitive for detecting FVIIa. Thus, we cannot confirm that FVIIa sensitivity of one-stage clotting assays for FVII:C is low when a rabbit thromboplastin and a non-adsorbed FVII-deficient plasma is used.
P Hellstern; H Beeck; A Fellhauer; A Fischer; B Faller-Stöckl
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Thrombosis research     Volume:  86     ISSN:  0049-3848     ISO Abbreviation:  Thromb. Res.     Publication Date:  1997 Jun 
Date Detail:
Created Date:  1997-09-15     Completed Date:  1997-09-15     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0326377     Medline TA:  Thromb Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  493-504     Citation Subset:  IM    
Institut für Transfusionsmedizin und Immunhämatologie, Klinikum der Stadt Ludwigshafen, Ludwigshafen, Germany.
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MeSH Terms
Blood Chemical Analysis
Factor VII / analysis*
Factor VIIa / analysis*
Middle Aged
Prothrombin / analysis*
Reference Values
Reproducibility of Results
Reg. No./Substance:
9001-25-6/Factor VII; 9001-26-7/Prothrombin; EC VIIa

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