| Measurement of erythrocyte carbonic anhydrase isozymes (CA-I and CA-II) in racehorses and riding horses. | |
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MedLine Citation:
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PMID: 15699596 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Equine carbonic anhydrase isozymes (CA-I and CA-II) were purified from erythrocytes by several column chromatography. Polyclonal anti-CA-I and anti-CA-II sera were produced in rabbits. Sensitive competitive enzyme-linked immunosorbent assays (ELISA) were established to determine the developmental changes in CA-I and CA-II levels in equine erythrocytes. Concentrations of CA-I and CA-II in erythrocytes from 150 clinically normal thoroughbreds (123 racehorses and 27 riding horses) were determined by ELISA. Mean (+/- SD) concentrations of CA-I and CA-II in racehorses were 1.70 +/- 0.48 and 0.94 +/- 0.13 mg/g hemoglobin (Hb), respectively. Mean concentrations of CA-I and CA-II in riding horses were 2.34 +/- 0.52 and 0.76 +/- 0.08 mg/g Hb, respectively. When the CA levels in racehorses and riding horses were compared, the CA-I level in riding horses was higher than that in racehorses (p=0.01). The CA-II level in racehorses was higher than that in riding horses (p=0.02). These data suggest that the levels of CA isozymes in erythrocytes of racehorses were influenced by chronic physical stress. The CA-I concentration in erythrocytes of 2-month-old horses was approximately 0.25 mg/g Hb. The CA-I level noticeably increased during the first year of life and approached normal adult levels by 2 years. The CA-II level decreased slightly with age, indicating different regulation of CA-I and CA-II expression during development. |
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Authors:
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Toshiho Nishita; Masako Takahasi; Tamae Kasuya; Kumi Matsui; Nobutsune Ichihara; Masaru Murakami; Masao Asari |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of veterinary medical science / the Japanese Society of Veterinary Science Volume: 67 ISSN: 0916-7250 ISO Abbreviation: J. Vet. Med. Sci. Publication Date: 2005 Jan |
Date Detail:
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Created Date: 2005-02-08 Completed Date: 2005-03-31 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9105360 Medline TA: J Vet Med Sci Country: Japan |
Other Details:
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Languages: eng Pagination: 63-7 Citation Subset: IM |
Affiliation:
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Laboratory of Veterinary Physiology 1, School of Veterinary Medicine, Azabu University, Fuchinobe, Sagamihara, Kanagawa, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aging
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blood Animals Carbonic Anhydrase I / blood* Carbonic Anhydrase II / blood* Enzyme-Linked Immunosorbent Assay / veterinary Erythrocytes / enzymology* Female Horses / blood*, physiology Male Physical Exertion / physiology* |
| Chemical | |
Reg. No./Substance:
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EC 4.2.1.-/Carbonic Anhydrase I; EC 4.2.1.-/Carbonic Anhydrase II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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