Document Detail

Measurement of erythrocyte arginase activity and the effect of lead on the activity in vitro.
MedLine Citation:
PMID:  2628839     Owner:  NLM     Status:  MEDLINE    
Measurement of arginase activity made by varying the arginine concentration and the reaction time clarified that the amount of urea formed by arginase peaked at the substrate concentration of 150 mmol/l, and increased linearly by 20 minutes. By using discs punched out from a dried blood filter paper prepared from identical human blood sample, the amount of formed urea showed linear increase up to 160 U/g.Hb. The coefficients of variation in ten measurements were 6.1% for the mean activity of 46.2 U/g.Hb and 2.1% for 152.1 U/g.Hb. No decreases in arginase activity were observed when two different dried blood filter papers were left for one week at room temperature. Intact erythrocytes were treated with various concentrations of lead from 0 to 5 mumols/l and their arginase activities were measured. The activity did not elevate linearly with the rise of lead concentration, but an almost diphasic elevation was observed. The arginase activity of erythrocytes treated with 5 mumols/l of lead was higher than that for untreated ones by 32%.
K Fukumoto; S Horiguchi; Y Nishikawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Osaka city medical journal     Volume:  35     ISSN:  0030-6096     ISO Abbreviation:  Osaka City Med J     Publication Date:  1989 Nov 
Date Detail:
Created Date:  1990-04-13     Completed Date:  1990-04-13     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0376413     Medline TA:  Osaka City Med J     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  101-8     Citation Subset:  IM    
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MeSH Terms
Arginase / blood*
Cells, Cultured
Erythrocytes / enzymology*
Lead / pharmacology*
Reg. No./Substance:
7439-92-1/Lead; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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