Document Detail


Measurement of erythrocyte arginase activity and the effect of lead on the activity in vitro.
MedLine Citation:
PMID:  2628839     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Measurement of arginase activity made by varying the arginine concentration and the reaction time clarified that the amount of urea formed by arginase peaked at the substrate concentration of 150 mmol/l, and increased linearly by 20 minutes. By using discs punched out from a dried blood filter paper prepared from identical human blood sample, the amount of formed urea showed linear increase up to 160 U/g.Hb. The coefficients of variation in ten measurements were 6.1% for the mean activity of 46.2 U/g.Hb and 2.1% for 152.1 U/g.Hb. No decreases in arginase activity were observed when two different dried blood filter papers were left for one week at room temperature. Intact erythrocytes were treated with various concentrations of lead from 0 to 5 mumols/l and their arginase activities were measured. The activity did not elevate linearly with the rise of lead concentration, but an almost diphasic elevation was observed. The arginase activity of erythrocytes treated with 5 mumols/l of lead was higher than that for untreated ones by 32%.
Authors:
K Fukumoto; S Horiguchi; Y Nishikawa
Related Documents :
25349469 - Arrays of high-aspect ratio microchannels for high-throughput isolation of circulating ...
8586789 - The efficacy of filters used in respiratory function apparatus.
9259989 - Preliminary investigation of the use of dried-blood spots for the assessment of in uter...
3781669 - Selective removal of pathogenetic macromolecules directly from whole blood by double fi...
1695479 - The detection of hemoglobin variants by isoelectrofocusing using edta-collected and fil...
7930069 - Group differences in measures of voice production and revised values of maximum airflow...
18560469 - Measurement of diffusion in flowing complex fluids.
17523089 - Clinical evaluation of the ca530-vet hematology analyzer for use in veterinary practice.
12464139 - Skin microvascular dilatation response to acetylcholine and sodium nitroprusside in per...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Osaka city medical journal     Volume:  35     ISSN:  0030-6096     ISO Abbreviation:  Osaka City Med J     Publication Date:  1989 Nov 
Date Detail:
Created Date:  1990-04-13     Completed Date:  1990-04-13     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0376413     Medline TA:  Osaka City Med J     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  101-8     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Arginase / blood*
Cells, Cultured
Erythrocytes / enzymology*
Humans
Lead / pharmacology*
Chemical
Reg. No./Substance:
7439-92-1/Lead; EC 3.5.3.1/Arginase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The effect of the group and individual characteristics of the vertebrae on their strength in experim...
Next Document:  Pulmonary embolization-induced thymus hyperplasia in rabbits.