Document Detail

Measurement of electrical bioimpedance for studying utilization of amino acids and peptides by Porphyromonas gingivalis, Fusobacterium nucleatum, and Treponema denticola.
MedLine Citation:
PMID:  8391864     Owner:  NLM     Status:  MEDLINE    
The growth response of Porphyromonas gingivalis, Fusobacterium nucleatum, and Treponema denticola to peptides (supplied as trypticase) and amino acids (supplied as casamino acids) was measured over 24 hours by monitoring changes in alternating-current conductivity at 37 degrees C. All species utilized peptides preferentially over amino acids. These results are consistent with those obtained previously with conventional growth-response experiments. The duration of the linear growth response to trypticase of P. gingivalis was 9.7 hours, whereas that of both F. nucleatum and T. denticola was > 24 hours. By contrast, there was more uniformity in the utilization of amino acids from the casamino acid mixture, which previously has been shown to be a poor growth substrate. Furthermore, subtle differences in growth patterns, such as the ability of F. nucleatum to metabolize its storage glycopolymers before utilizing amino acids, were clearly evident. The present method provides an excellent means of studying bacterial growth kinetics and delineating bacterial/substrate specificities of both synthetic and natural substrates.
H N Shah; S E Gharbia; M I Zhang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical infectious diseases : an official publication of the Infectious Diseases Society of America     Volume:  16 Suppl 4     ISSN:  1058-4838     ISO Abbreviation:  Clin. Infect. Dis.     Publication Date:  1993 Jun 
Date Detail:
Created Date:  1993-08-12     Completed Date:  1993-08-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9203213     Medline TA:  Clin Infect Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  S404-7     Citation Subset:  IM    
Department of Oral Biology, Dalhousie University, Halifax, Nova Scotia, Canada.
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MeSH Terms
Amino Acids / metabolism
Bacteriological Techniques / instrumentation
Culture Media
Electric Impedance
Fusobacterium nucleatum / growth & development,  metabolism*
Peptides / metabolism
Porphyromonas gingivalis / growth & development,  metabolism*
Treponema / growth & development,  metabolism*
Reg. No./Substance:
0/Amino Acids; 0/Culture Media; 0/Peptides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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